Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51927
Título: A recombinant dromedary antibody fragment (VHH or nanobody) directed against human Duffy antigen receptor for chemokines
Autores/as: Smolarek, Dorota
Hattab, Claude
Hassanzadeh-Ghassabeh, Gholamreza
Cochet, Sylvie
Gutierrez, Carlos 
De Brevern, Alexandre G.
Udomsangpetch, Rachanee
Picot, Julien
Grodecka, Magdalena
Wasniowska, Kazimiera
Muyldermans, Serge
Colin, Yves
Le Van Kim, Caroline
Czerwinski, Marcin
Bertrand, Olivier
Palabras clave: Plasmodium-Vivax
Monoclonal-Antibody
Hiv-1 Acquisition
Binding-Protein
Molecular-Basis, et al.
Fecha de publicación: 2010
Editor/a: 1420-682X
Publicación seriada: Cellular and Molecular Life Sciences 
Resumen: Fy blood group antigens are carried by the Duffy antigen receptor for chemokines (DARC), a red cells receptor for Plasmodium vivax broadly implicated in human health and diseases. Recombinant VHHs, or nanobodies, the smallest intact antigen binding fragment derivative from the heavy chain-only antibodies present in camelids, were prepared from a dromedary immunized against DARC N-terminal extracellular domain and selected for DARC binding. A described VHH, CA52, does recognize native DARC on cells. It inhibits P. vivax invasion of erythrocytes and displaces interleukin-8 bound to DARC. The targeted epitope overlaps the well-defined DARC Fy6 epitope. K (D) of CA52-DARC equilibrium is sub-nanomolar, hence ideal to develop diagnostic or therapeutic compounds. Immunocapture by immobilized CA52 yielded highly purified DARC from engineered K562 cells. This first report on a VHH with specificity for a red blood cell protein exemplifies VHHs' potentialities to target, to purify, and to modulate the function of cellular markers.
URI: http://hdl.handle.net/10553/51927
ISSN: 1420-682X
DOI: 10.1007/s00018-010-0387-6
Fuente: Cellular And Molecular Life Sciences[ISSN 1420-682X],v. 67 (19), p. 3371-3387
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