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Title: A recombinant dromedary antibody fragment (VHH or nanobody) directed against human Duffy antigen receptor for chemokines
Authors: Smolarek, Dorota
Hattab, Claude
Hassanzadeh-Ghassabeh, Gholamreza
Cochet, Sylvie
Gutierrez, Carlos 
De Brevern, Alexandre G.
Udomsangpetch, Rachanee
Picot, Julien
Grodecka, Magdalena
Wasniowska, Kazimiera
Muyldermans, Serge
Colin, Yves
Le Van Kim, Caroline
Czerwinski, Marcin
Bertrand, Olivier
Keywords: Plasmodium-Vivax
Hiv-1 Acquisition
Molecular-Basis, et al
Issue Date: 2010
Publisher: 1420-682X
Journal: Cellular and Molecular Life Sciences 
Abstract: Fy blood group antigens are carried by the Duffy antigen receptor for chemokines (DARC), a red cells receptor for Plasmodium vivax broadly implicated in human health and diseases. Recombinant VHHs, or nanobodies, the smallest intact antigen binding fragment derivative from the heavy chain-only antibodies present in camelids, were prepared from a dromedary immunized against DARC N-terminal extracellular domain and selected for DARC binding. A described VHH, CA52, does recognize native DARC on cells. It inhibits P. vivax invasion of erythrocytes and displaces interleukin-8 bound to DARC. The targeted epitope overlaps the well-defined DARC Fy6 epitope. K (D) of CA52-DARC equilibrium is sub-nanomolar, hence ideal to develop diagnostic or therapeutic compounds. Immunocapture by immobilized CA52 yielded highly purified DARC from engineered K562 cells. This first report on a VHH with specificity for a red blood cell protein exemplifies VHHs' potentialities to target, to purify, and to modulate the function of cellular markers.
ISSN: 1420-682X
DOI: 10.1007/s00018-010-0387-6
Source: Cellular And Molecular Life Sciences[ISSN 1420-682X],v. 67 (19), p. 3371-3387
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