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http://hdl.handle.net/10553/51927
Título: | A recombinant dromedary antibody fragment (VHH or nanobody) directed against human Duffy antigen receptor for chemokines | Autores/as: | Smolarek, Dorota Hattab, Claude Hassanzadeh-Ghassabeh, Gholamreza Cochet, Sylvie Gutierrez, Carlos De Brevern, Alexandre G. Udomsangpetch, Rachanee Picot, Julien Grodecka, Magdalena Wasniowska, Kazimiera Muyldermans, Serge Colin, Yves Le Van Kim, Caroline Czerwinski, Marcin Bertrand, Olivier |
Palabras clave: | Plasmodium-Vivax Monoclonal-Antibody Hiv-1 Acquisition Binding-Protein Molecular-Basis, et al. |
Fecha de publicación: | 2010 | Editor/a: | 1420-682X | Publicación seriada: | Cellular and Molecular Life Sciences | Resumen: | Fy blood group antigens are carried by the Duffy antigen receptor for chemokines (DARC), a red cells receptor for Plasmodium vivax broadly implicated in human health and diseases. Recombinant VHHs, or nanobodies, the smallest intact antigen binding fragment derivative from the heavy chain-only antibodies present in camelids, were prepared from a dromedary immunized against DARC N-terminal extracellular domain and selected for DARC binding. A described VHH, CA52, does recognize native DARC on cells. It inhibits P. vivax invasion of erythrocytes and displaces interleukin-8 bound to DARC. The targeted epitope overlaps the well-defined DARC Fy6 epitope. K (D) of CA52-DARC equilibrium is sub-nanomolar, hence ideal to develop diagnostic or therapeutic compounds. Immunocapture by immobilized CA52 yielded highly purified DARC from engineered K562 cells. This first report on a VHH with specificity for a red blood cell protein exemplifies VHHs' potentialities to target, to purify, and to modulate the function of cellular markers. | URI: | http://hdl.handle.net/10553/51927 | ISSN: | 1420-682X | DOI: | 10.1007/s00018-010-0387-6 | Fuente: | Cellular And Molecular Life Sciences[ISSN 1420-682X],v. 67 (19), p. 3371-3387 |
Colección: | Artículos |
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