|Title:||A recombinant dromedary antibody fragment (VHH or nanobody) directed against human Duffy antigen receptor for chemokines||Authors:||Smolarek, Dorota
De Brevern, Alexandre G.
Le Van Kim, Caroline
Molecular-Basis, et al
|Issue Date:||2010||Publisher:||1420-682X||Journal:||Cellular and Molecular Life Sciences||Abstract:||Fy blood group antigens are carried by the Duffy antigen receptor for chemokines (DARC), a red cells receptor for Plasmodium vivax broadly implicated in human health and diseases. Recombinant VHHs, or nanobodies, the smallest intact antigen binding fragment derivative from the heavy chain-only antibodies present in camelids, were prepared from a dromedary immunized against DARC N-terminal extracellular domain and selected for DARC binding. A described VHH, CA52, does recognize native DARC on cells. It inhibits P. vivax invasion of erythrocytes and displaces interleukin-8 bound to DARC. The targeted epitope overlaps the well-defined DARC Fy6 epitope. K (D) of CA52-DARC equilibrium is sub-nanomolar, hence ideal to develop diagnostic or therapeutic compounds. Immunocapture by immobilized CA52 yielded highly purified DARC from engineered K562 cells. This first report on a VHH with specificity for a red blood cell protein exemplifies VHHs' potentialities to target, to purify, and to modulate the function of cellular markers.||URI:||http://hdl.handle.net/10553/51927||ISSN:||1420-682X||DOI:||10.1007/s00018-010-0387-6||Source:||Cellular And Molecular Life Sciences[ISSN 1420-682X],v. 67 (19), p. 3371-3387|
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