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Title: Sporulation and sterilization method for axenic culture of Gelidium canariensis
Authors: Garcia-Jimenez, P 
Marian, FD
Rodrigo, M
Robaina, RR 
UNESCO Clasification: 241707 Algología (ficología)
Keywords: Red Alga
Axenic Culture
Gelidium Canariensis, et al
Issue Date: 1999
Journal: Journal of Biotechnology 
Conference: International Symposium on Marine Bioprocess Engineering 
Abstract: A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2 min). The branchlets were cultivated overnight in multiwell plates with 0.3 mi of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml(-1) each) and 0.1 g ml(-1) of GeO2 in liquid PES for 45 days, during which clusters of spores (85-100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis.
ISSN: 0168-1656
DOI: 10.1016/S0168-1656(99)00077-2
Source: Journal Of Biotechnology [ISSN 0168-1656], v. 70 (1-3), p. 227-229, (Abril 1999)
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