Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/72980
DC FieldValueLanguage
dc.contributor.authorGarcia-Jimenez, Pen_US
dc.contributor.authorMarian, FDen_US
dc.contributor.authorRodrigo, Men_US
dc.contributor.authorRobaina, RRen_US
dc.date.accessioned2020-06-04T12:10:36Z-
dc.date.available2020-06-04T12:10:36Z-
dc.date.issued1999en_US
dc.identifier.issn0168-1656en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/72980-
dc.description.abstractA sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2 min). The branchlets were cultivated overnight in multiwell plates with 0.3 mi of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml(-1) each) and 0.1 g ml(-1) of GeO2 in liquid PES for 45 days, during which clusters of spores (85-100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.sourceJournal Of Biotechnology [ISSN 0168-1656], v. 70 (1-3), p. 227-229, (Abril 1999)en_US
dc.subject241707 Algología (ficología)en_US
dc.subject.otherRed Algaen_US
dc.subject.otherGrowthen_US
dc.subject.otherDensityen_US
dc.subject.otherAxenic Cultureen_US
dc.subject.otherGelidium Canariensisen_US
dc.subject.otherRhodophytaen_US
dc.subject.otherSporelingsen_US
dc.titleSporulation and sterilization method for axenic culture of Gelidium canariensisen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.typeConferenceObjecten_US
dc.relation.conferenceInternational Symposium on Marine Bioprocess Engineering
dc.identifier.doi10.1016/S0168-1656(99)00077-2en_US
dc.identifier.scopus0033066484-
dc.identifier.isi000081185800027-
dc.contributor.authorscopusid6602635632-
dc.contributor.authorscopusid6602288996-
dc.contributor.authorscopusid8675279800-
dc.contributor.authorscopusid55885649200-
dc.description.lastpage229en_US
dc.identifier.issue1-3-
dc.description.firstpage227en_US
dc.relation.volume70en_US
dc.investigacionCienciasen_US
dc.type2Actas de congresosen_US
dc.contributor.daisngid1494106-
dc.contributor.daisngid10366954-
dc.contributor.daisngid23890914-
dc.contributor.daisngid1022386-
dc.description.numberofpages3en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Garcia-Jimenez, P-
dc.contributor.wosstandardWOS:Marian, FD-
dc.contributor.wosstandardWOS:Rodrigo, M-
dc.contributor.wosstandardWOS:Robaina, RR-
dc.date.coverdateAbril 1999en_US
dc.identifier.conferenceidevents120254-
dc.identifier.ulpgces
dc.description.jcr1,458
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.event.eventsstartdate08-11-1998-
crisitem.event.eventsenddate11-11-1998-
crisitem.author.deptBiología Integrativa y Recursos Biológicos-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptBiología-
crisitem.author.deptBiología Integrativa y Recursos Biológicos-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptBiología-
crisitem.author.orcid0000-0002-4732-0381-
crisitem.author.orcid0000-0003-4265-5809-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.fullNameGarcía Jiménez, M. Del Pilar-
crisitem.author.fullNameRobaina Romero, Rafael Juan-
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