|Title:||Functional activity of rat testicular cells in culture||Authors:||Centol, I.
Fanjul, L. F.
Ruiz de Galarreta, C. M.
|UNESCO Clasification:||32 Ciencias médicas
|Issue Date:||1988||Journal:||Biochemistry International||Abstract:||Testicular cells from adult hypophysectomized rats were cultured for 10 or 12 days, and the effect of treatment with hCG (10 ng/ml) on testosterone and progesterone production and the activity of the Leydig cell enzyme, 3 beta-hydroxysteroid dehydrogenase, were studied. Regardless of hormone treatment, on 4th day in culture a decline in the steroidogenic activity of cultured cells could be observed. Treatment with hCG resulted in stimulation of steroidogenesis on days 6 to 10 in culture, as measured by testosterone and progesterone production. Hormone treatment stimulated or inhibited the enzyme activity depending on the presence or absence in the culture medium of 10(-6) M spironolactone, an inhibitor of 17 alpha-hydroxylase, or an anti-androgen, cyproterone acetate.||URI:||http://hdl.handle.net/10553/45316||ISSN:||0158-5231||Source:||Biochemistry International [ISSN 0158-5231], v. 16 (2), p. 311-322|
|Appears in Collections:||Artículos|
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