Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53956
Title: Functional activity of rat testicular cells in culture
Authors: Centol, I.
Deniz, A.
Benitez, L.
Marrero, I. 
Fanjul, L. F. 
Ruiz de Galarreta, C. M.
UNESCO Clasification: 32 Ciencias médicas
2403 Bioquímica
Keywords: Functional activity
Testicular cells
Issue Date: 1988
Journal: Biochemistry International 
Abstract: Testicular cells from adult hypophysectomized rats were cultured for 10 or 12 days, and the effect of treatment with hCG (10 ng/ml) on testosterone and progesterone production and the activity of the Leydig cell enzyme, 3 beta-hydroxysteroid dehydrogenase, were studied. Regardless of hormone treatment, on 4th day in culture a decline in the steroidogenic activity of cultured cells could be observed. Treatment with hCG resulted in stimulation of steroidogenesis on days 6 to 10 in culture, as measured by testosterone and progesterone production. Hormone treatment stimulated or inhibited the enzyme activity depending on the presence or absence in the culture medium of 10(-6) M spironolactone, an inhibitor of 17 alpha-hydroxylase, or an anti-androgen, cyproterone acetate.
URI: http://hdl.handle.net/10553/45316
ISSN: 0158-5231
Source: Biochemistry International [ISSN 0158-5231], v. 16 (2), p. 311-322
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

6
checked on Dec 8, 2024

WEB OF SCIENCETM
Citations

6
checked on Feb 25, 2024

Page view(s)

36
checked on May 18, 2024

Google ScholarTM

Check


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.