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http://hdl.handle.net/10553/53956
Title: | Functional activity of rat testicular cells in culture | Authors: | Centol, I. Deniz, A. Benitez, L. Marrero, I. Fanjul, L. F. Ruiz de Galarreta, C. M. |
UNESCO Clasification: | 32 Ciencias médicas 2403 Bioquímica |
Keywords: | Functional activity Testicular cells |
Issue Date: | 1988 | Journal: | Biochemistry International | Abstract: | Testicular cells from adult hypophysectomized rats were cultured for 10 or 12 days, and the effect of treatment with hCG (10 ng/ml) on testosterone and progesterone production and the activity of the Leydig cell enzyme, 3 beta-hydroxysteroid dehydrogenase, were studied. Regardless of hormone treatment, on 4th day in culture a decline in the steroidogenic activity of cultured cells could be observed. Treatment with hCG resulted in stimulation of steroidogenesis on days 6 to 10 in culture, as measured by testosterone and progesterone production. Hormone treatment stimulated or inhibited the enzyme activity depending on the presence or absence in the culture medium of 10(-6) M spironolactone, an inhibitor of 17 alpha-hydroxylase, or an anti-androgen, cyproterone acetate. | URI: | http://hdl.handle.net/10553/45316 | ISSN: | 0158-5231 | Source: | Biochemistry International [ISSN 0158-5231], v. 16 (2), p. 311-322 |
Appears in Collections: | Artículos |
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