Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53534
Title: Partial characterization of a thyrotropin releasing hormone-sensitive glycosyl-phosphatidylinositol in pituitary lactotrophes
Authors: Benítez, Lourdes
Fanjul, Luisa F. 
Ruiz de Galarreta, C. M.
Quintana Aguiar, J. 
González Reyes, J.
Hernández, Inmaculada 
Santana Delgado, María del Pino 
Cabrera Oliva, Javier
Alonso Solís, R.
Estévez Rosas, Francisco
UNESCO Clasification: Investigación
Keywords: Thyrotropin releasing hormone
Glycosyl-phosphatidylinositol
Phosphatidylinositols
Inositol phosphoglycan
Issue Date: 1995
Publisher: 0304-3940
Journal: Neuroscience Letters 
Abstract: Metabolic labelling experiments performed with cultured pituitary lactotrophes revealed the presence of a glycosyl-phosphatidylinositol (GPtdlns) structurally related to GPtdlns lipids isolated from other cell types as demonstrated by: (i) metabolic incorporation of [3H]galactose, [3H]glucosamine and [3H]inositol into the polar inositolphosphoglycan moiety (InsPG) and [3H]myristate and [3H] palmitate into the diacylglycerol (DAG) backbone of GPtdlns; (ii) sensitivity of the [3H]labelled GPtdlns to nitrous acid deamination and; (iii) sensitivity of GPtdlns to phosphatidylinositol (Ptdlns)-specific phospholipase C (PLC) hydrolysis. In cultured pituitary cells labelled to isotopic steady state with 10 μCi/ml of [3H]glucosamine, treatment with hypothalamic TRH (10−6 M) induced a rapid and transient hydrolysis (ca. 50%) of the labelled GPtdlns. Moreover, as demonstrated in [3H]inositol labelled cells, treatment with thyrotropin releasing hormone (TRH) elicited the cleavage of [3H]GPtdlns in a similar manner, and this effect was followed by the phosphoinositide (Ptdlns, PtdInsP and PtdInsP2) hydrolysis 30 s later. These results suggest that the phosphodiesterase cleavage of GPtdlns could be an early event implicated in TRH action in pituitary lactotrophes.
URI: http://hdl.handle.net/10553/50825
ISSN: 0304-3940
DOI: 10.1016/0304-3940(95)11332-Q
Source: Neuroscience Letters [ISSN 0304-3940], v. 187 (1), p. 37-40
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