Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53534
DC FieldValueLanguage
dc.contributor.authorBenítez, Lourdesen_US
dc.contributor.authorFanjul, Luisa F.en_US
dc.contributor.authorRuiz de Galarreta, C. M.en_US
dc.contributor.authorQuintana Aguiar, J.en_US
dc.contributor.authorGonzález Reyes, J.en_US
dc.contributor.authorHernández, Inmaculadaen_US
dc.contributor.authorSantana Delgado, María del Pinoen_US
dc.contributor.authorCabrera Oliva, Javieren_US
dc.contributor.authorAlonso Solís, R.en_US
dc.contributor.authorEstévez Rosas, Franciscoen_US
dc.contributor.otherEstevez, Francisco-
dc.contributor.otherHernandez Gonzalez, Inmaculada-
dc.contributor.otherCabrera, Javier-
dc.contributor.otherQuintana, Jose-
dc.date.accessioned2019-02-04T17:05:10Z-
dc.date.available2019-02-04T17:05:10Z-
dc.date.issued1995en_US
dc.identifier.issn0304-3940en_US
dc.identifier.urihttp://hdl.handle.net/10553/50825-
dc.description.abstractMetabolic labelling experiments performed with cultured pituitary lactotrophes revealed the presence of a glycosyl-phosphatidylinositol (GPtdlns) structurally related to GPtdlns lipids isolated from other cell types as demonstrated by: (i) metabolic incorporation of [3H]galactose, [3H]glucosamine and [3H]inositol into the polar inositolphosphoglycan moiety (InsPG) and [3H]myristate and [3H] palmitate into the diacylglycerol (DAG) backbone of GPtdlns; (ii) sensitivity of the [3H]labelled GPtdlns to nitrous acid deamination and; (iii) sensitivity of GPtdlns to phosphatidylinositol (Ptdlns)-specific phospholipase C (PLC) hydrolysis. In cultured pituitary cells labelled to isotopic steady state with 10 μCi/ml of [3H]glucosamine, treatment with hypothalamic TRH (10−6 M) induced a rapid and transient hydrolysis (ca. 50%) of the labelled GPtdlns. Moreover, as demonstrated in [3H]inositol labelled cells, treatment with thyrotropin releasing hormone (TRH) elicited the cleavage of [3H]GPtdlns in a similar manner, and this effect was followed by the phosphoinositide (Ptdlns, PtdInsP and PtdInsP2) hydrolysis 30 s later. These results suggest that the phosphodiesterase cleavage of GPtdlns could be an early event implicated in TRH action in pituitary lactotrophes.en_US
dc.languageengen_US
dc.publisher0304-3940-
dc.relation.ispartofNeuroscience Lettersen_US
dc.sourceNeuroscience Letters [ISSN 0304-3940], v. 187 (1), p. 37-40en_US
dc.subjectInvestigaciónen_US
dc.subject.otherThyrotropin releasing hormoneen_US
dc.subject.otherGlycosyl-phosphatidylinositolen_US
dc.subject.otherPhosphatidylinositolsen_US
dc.subject.otherInositol phosphoglycanen_US
dc.titlePartial characterization of a thyrotropin releasing hormone-sensitive glycosyl-phosphatidylinositol in pituitary lactotrophesen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/0304-3940(95)11332-Qen_US
dc.identifier.scopus0028861799-
dc.identifier.isiA1995QP05500010-
dcterms.isPartOfNeuroscience Letters-
dcterms.sourceNeuroscience Letters[ISSN 0304-3940],v. 187 (1), p. 37-40-
dc.contributor.authorscopusid7004409333-
dc.contributor.authorscopusid7004158812-
dc.contributor.authorscopusid6506605794-
dc.contributor.authorscopusid7005928829-
dc.contributor.authorscopusid16167408800-
dc.contributor.authorscopusid7102922509-
dc.contributor.authorscopusid7006357982-
dc.contributor.authorscopusid7005997087-
dc.contributor.authorscopusid16940896600-
dc.contributor.authorscopusid7004224613-
dc.description.lastpage40-
dc.identifier.issue1-
dc.description.firstpage37-
dc.relation.volume187-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:A1995QP05500010-
dc.contributor.daisngid1155015-
dc.contributor.daisngid8966536
dc.contributor.daisngid1127140-
dc.contributor.daisngid1664323-
dc.contributor.daisngid6712290-
dc.contributor.daisngid369809-
dc.contributor.daisngid2446020-
dc.contributor.daisngid4535243-
dc.contributor.daisngid2353776-
dc.contributor.daisngid2241071
dc.contributor.daisngid1687789
dc.contributor.daisngid5111768-
dc.contributor.daisngid4633590-
dc.identifier.investigatorRIDK-5125-2014-
dc.identifier.investigatorRIDK-7776-2014-
dc.identifier.investigatorRIDL-9179-2014-
dc.identifier.investigatorRIDK-5709-2014-
dc.contributor.wosstandardWOS:BENITEZ, L
dc.contributor.wosstandardWOS:FANJUL, LF
dc.contributor.wosstandardWOS:DEGALARRETA, CMR
dc.contributor.wosstandardWOS:AGUIAR, JQ
dc.contributor.wosstandardWOS:REYES, JG
dc.contributor.wosstandardWOS:HERNANDEZ, I
dc.contributor.wosstandardWOS:DELGADO, PS
dc.contributor.wosstandardWOS:OLIVA, JC
dc.contributor.wosstandardWOS:SOLIS, RA
dc.contributor.wosstandardWOS:ROSAS, FE
dc.date.coverdateFebrero 1995
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.orcid0000-0001-8937-9034-
crisitem.author.orcid0000-0002-4093-2692-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
crisitem.author.fullNameQuintana Aguiar, José Martín-
crisitem.author.fullNameHernández González, Inmaculada Servanda-
crisitem.author.fullNameSantana Delgado, María Del Pino-
Appears in Collections:Artículos
Show simple item record

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.