|Title:||Overcoming matrix effects in electrospray: Quantitation of β-agonists in complex matrices by isotope dilution liquid chromatography-mass spectrometry using singly <sup>13</sup>C-labeled analogues||Authors:||González-Antuña, Ana
Domínguez-Romero, Juan C.
García-Reyes, Juan F.
García Alonso, J. Ignacio
|UNESCO Clasification:||32 Ciencias médicas
230103 Análisis cromatográfico
230110 Espectroscopia de masas
|Issue Date:||2013||Journal:||Journal of Chromatography A||Abstract:||In this work, the implementation of isotope dilution mass spectrometry (IDMS) using minimal labeling and isotope pattern deconvolution (IPD) is evaluated as a strategy for the minimization of matrix effects during trace determination of β2-agonists in complex matrices by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). First, the parameters affecting the measurement of isotopic composition of organic compounds by liquid chromatography electrospray ionization high resolution mass spectrometry with a time-of-flight analyzer were evaluated using as a case of study three different β2-agonists: clenbuterol, clenproperol and brombuterol. Then, a calibration graph-free IDMS methodology was evaluated in order to overcome matrix effects in LC-ESI-MS in complex samples. In this procedure singly (13)C-labeled analogues of clenbuterol, clenproperol and brombuterol were employed in combination with IPD. Using this approach accurate and precise results were obtained in the simultaneous quantification of β2-agonists in human urine and bovine liver, even at the sub ngg(-1) and particularly in spite of the previously reported matrix effects. Recovery rates in the range of 97-114% in fortified human urine and from 95% to 111% in fortified bovine liver were obtained with RSD (%) of independent recovery experiments always lower than 6%. These results demonstrate that the proposed methodology based on the use of (13)C1-labeled standards and IPD is a reliable approach for accurate LC-MS quantitation of small molecules and compatible with full-scan high-resolution mass spectrometry.||URI:||http://hdl.handle.net/10553/45332||ISSN:||0021-9673||DOI:||10.1016/j.chroma.2013.02.074||Source:||Journal of Chromatography A [ISSN 0021-9673],v. 1288, p. 40-47 (Mayo 2013)|
|Appears in Collections:||Artículos|
checked on May 16, 2021
checked on May 16, 2021
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.