Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/45332
DC Field | Value | Language |
---|---|---|
dc.contributor.author | González-Antuña, Ana | en_US |
dc.contributor.author | Domínguez-Romero, Juan C. | en_US |
dc.contributor.author | García-Reyes, Juan F. | en_US |
dc.contributor.author | Rodríguez-González, Pablo | en_US |
dc.contributor.author | Centineo, Giuseppe | en_US |
dc.contributor.author | García Alonso, J. Ignacio | en_US |
dc.contributor.author | Molina-Díaz, Antonio | en_US |
dc.date.accessioned | 2018-11-22T09:00:54Z | - |
dc.date.available | 2018-11-22T09:00:54Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.issn | 0021-9673 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/45332 | - |
dc.description.abstract | In this work, the implementation of isotope dilution mass spectrometry (IDMS) using minimal labeling and isotope pattern deconvolution (IPD) is evaluated as a strategy for the minimization of matrix effects during trace determination of β2-agonists in complex matrices by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). First, the parameters affecting the measurement of isotopic composition of organic compounds by liquid chromatography electrospray ionization high resolution mass spectrometry with a time-of-flight analyzer were evaluated using as a case of study three different β2-agonists: clenbuterol, clenproperol and brombuterol. Then, a calibration graph-free IDMS methodology was evaluated in order to overcome matrix effects in LC-ESI-MS in complex samples. In this procedure singly (13)C-labeled analogues of clenbuterol, clenproperol and brombuterol were employed in combination with IPD. Using this approach accurate and precise results were obtained in the simultaneous quantification of β2-agonists in human urine and bovine liver, even at the sub ngg(-1) and particularly in spite of the previously reported matrix effects. Recovery rates in the range of 97-114% in fortified human urine and from 95% to 111% in fortified bovine liver were obtained with RSD (%) of independent recovery experiments always lower than 6%. These results demonstrate that the proposed methodology based on the use of (13)C1-labeled standards and IPD is a reliable approach for accurate LC-MS quantitation of small molecules and compatible with full-scan high-resolution mass spectrometry. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Chromatography A | en_US |
dc.source | Journal of Chromatography A [ISSN 0021-9673],v. 1288, p. 40-47 (Mayo 2013) | en_US |
dc.subject | 32 Ciencias médicas | en_US |
dc.subject | 230103 Análisis cromatográfico | en_US |
dc.subject | 230110 Espectroscopia de masas | en_US |
dc.subject.other | Electrospray | en_US |
dc.subject.other | β-agonists | en_US |
dc.subject.other | Chromatography-mass spectrometry | en_US |
dc.title | Overcoming matrix effects in electrospray: Quantitation of β-agonists in complex matrices by isotope dilution liquid chromatography-mass spectrometry using singly <sup>13</sup>C-labeled analogues | en_US |
dc.type | info:eu-repo/semantics/article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.chroma.2013.02.074 | en_US |
dc.identifier.scopus | 84876077293 | - |
dc.contributor.authorscopusid | 36058862700 | - |
dc.contributor.authorscopusid | 53263319000 | - |
dc.contributor.authorscopusid | 56360245800 | - |
dc.contributor.authorscopusid | 6603228633 | - |
dc.contributor.authorscopusid | 35557054000 | - |
dc.contributor.authorscopusid | 7005508838 | - |
dc.contributor.authorscopusid | 57190577987 | - |
dc.description.lastpage | 47 | en_US |
dc.description.firstpage | 40 | en_US |
dc.relation.volume | 1288 | en_US |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.description.numberofpages | 8 | en_US |
dc.utils.revision | Sí | en_US |
dc.date.coverdate | Mayo 2013 | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.contributor.buulpgc | BU-MED | en_US |
dc.description.sjr | 2,017 | |
dc.description.jcr | 4,258 | |
dc.description.sjrq | Q1 | |
dc.description.jcrq | Q1 | |
dc.description.scie | SCIE | |
item.grantfulltext | none | - |
item.fulltext | Sin texto completo | - |
crisitem.author.fullName | González Antuña, Ana | - |
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