Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/45332
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dc.contributor.authorGonzález-Antuña, Anaen_US
dc.contributor.authorDomínguez-Romero, Juan C.en_US
dc.contributor.authorGarcía-Reyes, Juan F.en_US
dc.contributor.authorRodríguez-González, Pabloen_US
dc.contributor.authorCentineo, Giuseppeen_US
dc.contributor.authorGarcía Alonso, J. Ignacioen_US
dc.contributor.authorMolina-Díaz, Antonioen_US
dc.date.accessioned2018-11-22T09:00:54Z-
dc.date.available2018-11-22T09:00:54Z-
dc.date.issued2013en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://hdl.handle.net/10553/45332-
dc.description.abstractIn this work, the implementation of isotope dilution mass spectrometry (IDMS) using minimal labeling and isotope pattern deconvolution (IPD) is evaluated as a strategy for the minimization of matrix effects during trace determination of β2-agonists in complex matrices by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). First, the parameters affecting the measurement of isotopic composition of organic compounds by liquid chromatography electrospray ionization high resolution mass spectrometry with a time-of-flight analyzer were evaluated using as a case of study three different β2-agonists: clenbuterol, clenproperol and brombuterol. Then, a calibration graph-free IDMS methodology was evaluated in order to overcome matrix effects in LC-ESI-MS in complex samples. In this procedure singly (13)C-labeled analogues of clenbuterol, clenproperol and brombuterol were employed in combination with IPD. Using this approach accurate and precise results were obtained in the simultaneous quantification of β2-agonists in human urine and bovine liver, even at the sub ngg(-1) and particularly in spite of the previously reported matrix effects. Recovery rates in the range of 97-114% in fortified human urine and from 95% to 111% in fortified bovine liver were obtained with RSD (%) of independent recovery experiments always lower than 6%. These results demonstrate that the proposed methodology based on the use of (13)C1-labeled standards and IPD is a reliable approach for accurate LC-MS quantitation of small molecules and compatible with full-scan high-resolution mass spectrometry.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Chromatography Aen_US
dc.sourceJournal of Chromatography A [ISSN 0021-9673],v. 1288, p. 40-47 (Mayo 2013)en_US
dc.subject32 Ciencias médicasen_US
dc.subject230103 Análisis cromatográficoen_US
dc.subject230110 Espectroscopia de masasen_US
dc.subject.otherElectrosprayen_US
dc.subject.otherβ-agonistsen_US
dc.subject.otherChromatography-mass spectrometryen_US
dc.titleOvercoming matrix effects in electrospray: Quantitation of β-agonists in complex matrices by isotope dilution liquid chromatography-mass spectrometry using singly <sup>13</sup>C-labeled analoguesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.chroma.2013.02.074en_US
dc.identifier.scopus84876077293-
dc.contributor.authorscopusid36058862700-
dc.contributor.authorscopusid53263319000-
dc.contributor.authorscopusid56360245800-
dc.contributor.authorscopusid6603228633-
dc.contributor.authorscopusid35557054000-
dc.contributor.authorscopusid7005508838-
dc.contributor.authorscopusid57190577987-
dc.description.lastpage47en_US
dc.description.firstpage40en_US
dc.relation.volume1288en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.date.coverdateMayo 2013en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr2,017
dc.description.jcr4,258
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.fullNameGonzález Antuña, Ana-
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