Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/45332
Title: | Overcoming matrix effects in electrospray: Quantitation of β-agonists in complex matrices by isotope dilution liquid chromatography-mass spectrometry using singly <sup>13</sup>C-labeled analogues | Authors: | González-Antuña, Ana Domínguez-Romero, Juan C. García-Reyes, Juan F. Rodríguez-González, Pablo Centineo, Giuseppe García Alonso, J. Ignacio Molina-Díaz, Antonio |
UNESCO Clasification: | 32 Ciencias médicas 230103 Análisis cromatográfico 230110 Espectroscopia de masas |
Keywords: | Electrospray β-agonists Chromatography-mass spectrometry |
Issue Date: | 2013 | Journal: | Journal of Chromatography A | Abstract: | In this work, the implementation of isotope dilution mass spectrometry (IDMS) using minimal labeling and isotope pattern deconvolution (IPD) is evaluated as a strategy for the minimization of matrix effects during trace determination of β2-agonists in complex matrices by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). First, the parameters affecting the measurement of isotopic composition of organic compounds by liquid chromatography electrospray ionization high resolution mass spectrometry with a time-of-flight analyzer were evaluated using as a case of study three different β2-agonists: clenbuterol, clenproperol and brombuterol. Then, a calibration graph-free IDMS methodology was evaluated in order to overcome matrix effects in LC-ESI-MS in complex samples. In this procedure singly (13)C-labeled analogues of clenbuterol, clenproperol and brombuterol were employed in combination with IPD. Using this approach accurate and precise results were obtained in the simultaneous quantification of β2-agonists in human urine and bovine liver, even at the sub ngg(-1) and particularly in spite of the previously reported matrix effects. Recovery rates in the range of 97-114% in fortified human urine and from 95% to 111% in fortified bovine liver were obtained with RSD (%) of independent recovery experiments always lower than 6%. These results demonstrate that the proposed methodology based on the use of (13)C1-labeled standards and IPD is a reliable approach for accurate LC-MS quantitation of small molecules and compatible with full-scan high-resolution mass spectrometry. | URI: | http://hdl.handle.net/10553/45332 | ISSN: | 0021-9673 | DOI: | 10.1016/j.chroma.2013.02.074 | Source: | Journal of Chromatography A [ISSN 0021-9673],v. 1288, p. 40-47 (Mayo 2013) |
Appears in Collections: | Artículos |
SCOPUSTM
Citations
50
checked on Nov 17, 2024
WEB OF SCIENCETM
Citations
48
checked on Nov 17, 2024
Page view(s)
59
checked on Oct 31, 2024
Google ScholarTM
Check
Altmetric
Share
Export metadata
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.