Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/43406
Title: Demonstration of rat CAR bacillus using a labelled streptavidin biotin (LSAB) method
Authors: Orós Montón, Jorge Ignacio 
Matsushita, Satoru
Rodríguez Navarro, José Luis 
Rodríguez Guisado, Francisco 
Fernández Rodríguez, Antonio Jesús 
UNESCO Clasification: 310907 Patología
Keywords: bacillus
inmunohistochemistry
rat
Issue Date: 1996
Publisher: 0916-7250
Journal: Journal of Veterinary Medical Science 
Abstract: Immunohistochemical detection of rat CAR bacillus antigen in paraffin-embedded experimentally infected rat lungs, using an immunoperoxidase technique based on the labelled streptavidin biotin (LSAB) method and 3-amino-9-ethylcarbazole (AEC) as substrate is described in this paper. The pattern of immunostaining was confined to the ciliated bronchial epithelium and the specificity of this technique was confirmed. The use of AEC as substrate was evaluated more efficient than diaminobenzidine (DAB). The usefulness of this immunoperoxidase technique for the detection of CAR bacillus in rats and its advantages compared to the indirect immunofluorescence (IF) are discussed.
URI: http://hdl.handle.net/10553/43406
ISSN: 0916-7250
DOI: 10.1292/jvms.58.12_1219
Source: Journal Of Veterinary Medical Science [ISSN 0916-7250], v. 58 (12), p. 1219-1221
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