Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/43406
Título: Demonstration of rat CAR bacillus using a labelled streptavidin biotin (LSAB) method
Autores/as: Orós Montón, Jorge Ignacio 
Matsushita, Satoru
Rodríguez Navarro, José Luis 
Rodríguez Guisado, Francisco 
Fernández Rodríguez, Antonio Jesús 
Clasificación UNESCO: 310907 Patología
Palabras clave: bacillus
inmunohistochemistry
rat
Fecha de publicación: 1996
Editor/a: 0916-7250
Publicación seriada: Journal of Veterinary Medical Science 
Resumen: Immunohistochemical detection of rat CAR bacillus antigen in paraffin-embedded experimentally infected rat lungs, using an immunoperoxidase technique based on the labelled streptavidin biotin (LSAB) method and 3-amino-9-ethylcarbazole (AEC) as substrate is described in this paper. The pattern of immunostaining was confined to the ciliated bronchial epithelium and the specificity of this technique was confirmed. The use of AEC as substrate was evaluated more efficient than diaminobenzidine (DAB). The usefulness of this immunoperoxidase technique for the detection of CAR bacillus in rats and its advantages compared to the indirect immunofluorescence (IF) are discussed.
URI: http://hdl.handle.net/10553/43406
ISSN: 0916-7250
DOI: 10.1292/jvms.58.12_1219
Fuente: Journal Of Veterinary Medical Science [ISSN 0916-7250], v. 58 (12), p. 1219-1221
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