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http://hdl.handle.net/10553/43406
Título: | Demonstration of rat CAR bacillus using a labelled streptavidin biotin (LSAB) method | Autores/as: | Orós Montón, Jorge Ignacio Matsushita, Satoru Rodríguez Navarro, José Luis Rodríguez Guisado, Francisco Fernández Rodríguez, Antonio Jesús |
Clasificación UNESCO: | 310907 Patología | Palabras clave: | bacillus inmunohistochemistry rat |
Fecha de publicación: | 1996 | Editor/a: | 0916-7250 | Publicación seriada: | Journal of Veterinary Medical Science | Resumen: | Immunohistochemical detection of rat CAR bacillus antigen in paraffin-embedded experimentally infected rat lungs, using an immunoperoxidase technique based on the labelled streptavidin biotin (LSAB) method and 3-amino-9-ethylcarbazole (AEC) as substrate is described in this paper. The pattern of immunostaining was confined to the ciliated bronchial epithelium and the specificity of this technique was confirmed. The use of AEC as substrate was evaluated more efficient than diaminobenzidine (DAB). The usefulness of this immunoperoxidase technique for the detection of CAR bacillus in rats and its advantages compared to the indirect immunofluorescence (IF) are discussed. | URI: | http://hdl.handle.net/10553/43406 | ISSN: | 0916-7250 | DOI: | 10.1292/jvms.58.12_1219 | Fuente: | Journal Of Veterinary Medical Science [ISSN 0916-7250], v. 58 (12), p. 1219-1221 |
Colección: | Artículos |
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