Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/74180
Título: DNA-printing: utilization of a standard inkjet printer for the transfer of nucleic acids to solid supports
Autores/as: Goldmann, Torsten
González, Juan Santiago
Clasificación UNESCO: 2403 Bioquímica
Palabras clave: Nucleic Acids
Inkjet Printer
Hybridization
Blotting
Membranes
Fecha de publicación: 2000
Publicación seriada: Journal of Biochemical and Biophysical Methods 
Resumen: The use of total cDNA as a probe for hybridization enables the transcription level of a large number of genes to be analyzed at the same time. Some effort has been spent to develop high density gene arrays on different solid supports to facilitate this hybridization. We achieved a high resolution by utilizing inkjet printer technology as a useful alternative to blotting the target genes onto a membrane. By the use of an ordinary inkjet printer model we show that it is possible to print DNA onto hybridization membranes and hybridize using either specific genes or total cDNA as probes. The high resolution of these prints (300 dpi) might be used in the future to construct complex micro-arrays to analyze simultaneously large numbers of genes.
URI: http://hdl.handle.net/10553/74180
ISSN: 0165-022X
DOI: 10.1016/S0165-022X(99)00049-4
Fuente: Journal of Biochemical and Biophysical Methods [ISSN 0165-022X], v. 42 (3), p. 105-110, (Marzo 2000)
Colección:Artículos
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