Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/74180
Campo DC Valoridioma
dc.contributor.authorGoldmann, Torstenen_US
dc.contributor.authorGonzález, Juan Santiagoen_US
dc.date.accessioned2020-08-21T21:33:23Z-
dc.date.available2020-08-21T21:33:23Z-
dc.date.issued2000en_US
dc.identifier.issn0165-022Xen_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/74180-
dc.description.abstractThe use of total cDNA as a probe for hybridization enables the transcription level of a large number of genes to be analyzed at the same time. Some effort has been spent to develop high density gene arrays on different solid supports to facilitate this hybridization. We achieved a high resolution by utilizing inkjet printer technology as a useful alternative to blotting the target genes onto a membrane. By the use of an ordinary inkjet printer model we show that it is possible to print DNA onto hybridization membranes and hybridize using either specific genes or total cDNA as probes. The high resolution of these prints (300 dpi) might be used in the future to construct complex micro-arrays to analyze simultaneously large numbers of genes.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Biochemical and Biophysical Methodsen_US
dc.sourceJournal of Biochemical and Biophysical Methods [ISSN 0165-022X], v. 42 (3), p. 105-110, (Marzo 2000)en_US
dc.subject2403 Bioquímicaen_US
dc.subject.otherNucleic Acidsen_US
dc.subject.otherInkjet Printeren_US
dc.subject.otherHybridizationen_US
dc.subject.otherBlottingen_US
dc.subject.otherMembranesen_US
dc.titleDNA-printing: utilization of a standard inkjet printer for the transfer of nucleic acids to solid supportsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/S0165-022X(99)00049-4en_US
dc.identifier.isi000085741000003-
dc.description.lastpage110en_US
dc.identifier.issue3-
dc.description.firstpage105en_US
dc.relation.volume42en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid142585-
dc.contributor.daisngid15527542-
dc.description.numberofpages6en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Goldmann, T-
dc.contributor.wosstandardWOS:Gonzalez, JS-
dc.date.coverdateMarzo 2000en_US
dc.identifier.ulpgces
dc.description.jcr0,926
dc.description.jcrqQ3
item.grantfulltextnone-
item.fulltextSin texto completo-
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