Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77013
Title: Genetic variability in cysteine protease genes of Haemonchus contortus
Authors: Ruiz Reyes, Antonio 
Molina Caballero, José Manuel 
Njue, A.
Prichard, R. K.
UNESCO Clasification: 240112 Parasitología animal
Keywords: Haemonchus contortus
Cysteine proteases
Genetic variation
SSCP
Issue Date: 2004
Journal: Parasitology 
Abstract: To increase the existent genetic variability in cysteine proteases, a polymorphism study was performed in Haemonchus contortus by comparing 2 different strains of the parasite: North American (NA) and Spanish (SP) strains. For this purpose, the polymorphism of 5 previously reported genes (AC-1, AC-3, AC-4, AC-5 and GCP-7) were analysed by PCR-SSCP and sequencing procedures. Based oil the SSCP results, a total of 20 different alleles were identified for the 3 loci assessed. Except locus AC-5, all the loci were polymorphic. Loci AC-1, AC-3, AC-4 GCP-7 showed 5, 8, 2 and 4 alleles, respectively. The allelic frequencies ranged from 0.0070 to 0.8560 and were significantly different between strains. In addition, nucleotide diversity analyses showed a significant variation within and between strains. The variations in the nucleotide sequence of the different alleles were translated in some cases into changes in the amino acid sequence. Evidence of genetic variability in cysteine proteases from two different strains of H. contortus for the same set of genes had not been previously reported.
URI: http://hdl.handle.net/10553/77013
ISSN: 0031-1820
DOI: 10.1017/S0031182004004998
Source: Parasitology [ISSN 0031-1820], v. 128 (5), p. 549-559, (Mayo 2004)
Appears in Collections:Artículos
Thumbnail
pdf
Adobe PDF (154,09 kB)
Show full item record

SCOPUSTM   
Citations

9
checked on Sep 29, 2024

WEB OF SCIENCETM
Citations

8
checked on Sep 29, 2024

Page view(s)

124
checked on Feb 25, 2024

Download(s)

141
checked on Feb 25, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.