Please use this identifier to cite or link to this item: https://accedacris.ulpgc.es/handle/10553/77013
Título: Genetic variability in cysteine protease genes of Haemonchus contortus
Autores/as: Ruiz Reyes, Antonio 
Molina Caballero, José Manuel 
Njue, A.
Prichard, R. K.
Clasificación UNESCO: 240112 Parasitología animal
Palabras clave: Haemonchus contortus
Cysteine proteases
Genetic variation
SSCP
Fecha de publicación: 2004
Publicación seriada: Parasitology 
Resumen: To increase the existent genetic variability in cysteine proteases, a polymorphism study was performed in Haemonchus contortus by comparing 2 different strains of the parasite: North American (NA) and Spanish (SP) strains. For this purpose, the polymorphism of 5 previously reported genes (AC-1, AC-3, AC-4, AC-5 and GCP-7) were analysed by PCR-SSCP and sequencing procedures. Based oil the SSCP results, a total of 20 different alleles were identified for the 3 loci assessed. Except locus AC-5, all the loci were polymorphic. Loci AC-1, AC-3, AC-4 GCP-7 showed 5, 8, 2 and 4 alleles, respectively. The allelic frequencies ranged from 0.0070 to 0.8560 and were significantly different between strains. In addition, nucleotide diversity analyses showed a significant variation within and between strains. The variations in the nucleotide sequence of the different alleles were translated in some cases into changes in the amino acid sequence. Evidence of genetic variability in cysteine proteases from two different strains of H. contortus for the same set of genes had not been previously reported.
URI: https://accedacris.ulpgc.es/handle/10553/77013
ISSN: 0031-1820
DOI: 10.1017/S0031182004004998
Source: Parasitology [ISSN 0031-1820], v. 128 (5), p. 549-559, (Mayo 2004)
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