Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77013
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dc.contributor.authorRuiz Reyes, Antonioen_US
dc.contributor.authorMolina Caballero, José Manuelen_US
dc.contributor.authorNjue, A.en_US
dc.contributor.authorPrichard, R. K.en_US
dc.date.accessioned2021-01-04T09:31:21Z-
dc.date.available2021-01-04T09:31:21Z-
dc.date.issued2004en_US
dc.identifier.issn0031-1820en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/77013-
dc.description.abstractTo increase the existent genetic variability in cysteine proteases, a polymorphism study was performed in Haemonchus contortus by comparing 2 different strains of the parasite: North American (NA) and Spanish (SP) strains. For this purpose, the polymorphism of 5 previously reported genes (AC-1, AC-3, AC-4, AC-5 and GCP-7) were analysed by PCR-SSCP and sequencing procedures. Based oil the SSCP results, a total of 20 different alleles were identified for the 3 loci assessed. Except locus AC-5, all the loci were polymorphic. Loci AC-1, AC-3, AC-4 GCP-7 showed 5, 8, 2 and 4 alleles, respectively. The allelic frequencies ranged from 0.0070 to 0.8560 and were significantly different between strains. In addition, nucleotide diversity analyses showed a significant variation within and between strains. The variations in the nucleotide sequence of the different alleles were translated in some cases into changes in the amino acid sequence. Evidence of genetic variability in cysteine proteases from two different strains of H. contortus for the same set of genes had not been previously reported.en_US
dc.languageengen_US
dc.relation.ispartofParasitologyen_US
dc.sourceParasitology [ISSN 0031-1820], v. 128 (5), p. 549-559, (Mayo 2004)en_US
dc.subject240112 Parasitología animalen_US
dc.subject.otherHaemonchus contortusen_US
dc.subject.otherCysteine proteasesen_US
dc.subject.otherGenetic variationen_US
dc.subject.otherSSCPen_US
dc.titleGenetic variability in cysteine protease genes of Haemonchus contortusen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1017/S0031182004004998en_US
dc.identifier.scopus2542498963-
dc.identifier.isi000221650700009-
dc.contributor.authorscopusid57201387360-
dc.contributor.authorscopusid55731962600-
dc.contributor.authorscopusid57197797025-
dc.contributor.authorscopusid23055977100-
dc.description.lastpage559en_US
dc.description.firstpage549en_US
dc.relation.volume128en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid1497623-
dc.contributor.daisngid742292-
dc.contributor.daisngid6969526-
dc.contributor.daisngid52000-
dc.description.numberofpages11en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Ruiz, A-
dc.contributor.wosstandardWOS:Molina, JM-
dc.contributor.wosstandardWOS:Njue, A-
dc.contributor.wosstandardWOS:Prichard, RK-
dc.date.coverdateMayo 2004en_US
dc.identifier.ulpgcen_US
dc.description.jcr1,685
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-0668-5496-
crisitem.author.orcid0000-0002-8338-2512-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.fullNameRuiz Reyes, Antonio-
crisitem.author.fullNameMolina Caballero, José Manuel-
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