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Title: Echinococcus multilocularis laminated-layer components and the E14t 14-3-3 recombinant protein decrease NO production by activated rat macrophages in vitro
Authors: Andrade, M. Amparo
Siles-Lucas, Mar
Espinoza, Elsa
Pérez Arellano, José Luis 
Gottstein, Bruno
Muro, Antonio
UNESCO Clasification: 32 Ciencias médicas
320712 Parasitología
Keywords: Nitric-oxide
Alveolar macrophages
Echinococcus granulosus
Echinococcus multilocularis
Issue Date: 2004
Journal: Nitric Oxide - Biology and Chemistry 
Abstract: Echinococcus multiloeularis and Echinococcus granulosus cause alveolar and cystic (unilocular) echinococcosis, respectively, in humans and animals. It is known that these parasites can affect, among other molecules, nitric oxide (NO) production by periparasitic host cells. Nevertheless, detailed dissection of parasite components specifically affecting cell NO production has not been done to date. We compare the effect of E. granulosus and E. multilocularis defined metacestode structural (laminated-layer associated) and metabolic (14-3-3 protein, potentially related with E. multilocularis metacestode tumor-like growth) components on the NO production by rat alveolar macrophages in vitro. Our results showed that none of these antigens could stimulate macrophage NO production in vitro. However, a reversed effect of some Echinococcus antigens on NO in vitro production was found when cells were previously exposed to LPS stimulation. This inhibitory effect was found when E. multilocularis laminated-layer (LL) or cyst wall (CW) soluble components from both species were used. Pre-stimulation of cells with LPS also resulted in a strong, dose-dependent reduction of NO and iNOS mRNA production after incubation of cells with the E14t protein. Thus, the E. multilocularis 14-3-3 protein appears to be one of the components accounting for the suppressive effect of the CW and LL metacestode extracts.
ISSN: 1089-8603
DOI: 10.1016/j.niox.2004.03.002
Source: Nitric Oxide-Biology and Chemistry [ISSN 1089-8603], v. 10 (3), p. 150-155, 2004
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