Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/76959
DC FieldValueLanguage
dc.contributor.authorAndrade, M. Amparoen_US
dc.contributor.authorSiles-Lucas, Maren_US
dc.contributor.authorEspinoza, Elsaen_US
dc.contributor.authorPérez Arellano, José Luisen_US
dc.contributor.authorGottstein, Brunoen_US
dc.contributor.authorMuro, Antonioen_US
dc.date.accessioned2020-12-23T12:20:27Z-
dc.date.available2020-12-23T12:20:27Z-
dc.date.issued2004en_US
dc.identifier.issn1089-8603en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/76959-
dc.description.abstractEchinococcus multiloeularis and Echinococcus granulosus cause alveolar and cystic (unilocular) echinococcosis, respectively, in humans and animals. It is known that these parasites can affect, among other molecules, nitric oxide (NO) production by periparasitic host cells. Nevertheless, detailed dissection of parasite components specifically affecting cell NO production has not been done to date. We compare the effect of E. granulosus and E. multilocularis defined metacestode structural (laminated-layer associated) and metabolic (14-3-3 protein, potentially related with E. multilocularis metacestode tumor-like growth) components on the NO production by rat alveolar macrophages in vitro. Our results showed that none of these antigens could stimulate macrophage NO production in vitro. However, a reversed effect of some Echinococcus antigens on NO in vitro production was found when cells were previously exposed to LPS stimulation. This inhibitory effect was found when E. multilocularis laminated-layer (LL) or cyst wall (CW) soluble components from both species were used. Pre-stimulation of cells with LPS also resulted in a strong, dose-dependent reduction of NO and iNOS mRNA production after incubation of cells with the E14t protein. Thus, the E. multilocularis 14-3-3 protein appears to be one of the components accounting for the suppressive effect of the CW and LL metacestode extracts.en_US
dc.languageengen_US
dc.relation.ispartofNitric Oxide - Biology and Chemistryen_US
dc.sourceNitric Oxide-Biology and Chemistry [ISSN 1089-8603], v. 10 (3), p. 150-155, 2004en_US
dc.subject32 Ciencias médicasen_US
dc.subject320712 Parasitologíaen_US
dc.subject.otherNitric-oxideen_US
dc.subject.otherAlveolar macrophagesen_US
dc.subject.otherEchinococcus granulosusen_US
dc.subject.otherEchinococcus multilocularisen_US
dc.subject.other14-3-3en_US
dc.titleEchinococcus multilocularis laminated-layer components and the E14t 14-3-3 recombinant protein decrease NO production by activated rat macrophages in vitroen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.niox.2004.03.002en_US
dc.identifier.scopus2442668200-
dc.identifier.isi000221974200004-
dc.contributor.authorscopusid36929356100-
dc.contributor.authorscopusid15045746000-
dc.contributor.authorscopusid7004660805-
dc.contributor.authorscopusid7007078140-
dc.contributor.authorscopusid7005746619-
dc.contributor.authorscopusid7006690116-
dc.identifier.eissn1089-8611-
dc.description.lastpage155en_US
dc.identifier.issue3-
dc.description.firstpage150en_US
dc.relation.volume10en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid21575803-
dc.contributor.daisngid563367-
dc.contributor.daisngid5314492-
dc.contributor.daisngid445671-
dc.contributor.daisngid36863-
dc.contributor.daisngid409562-
dc.description.numberofpages6en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Andrade, MA-
dc.contributor.wosstandardWOS:Siles-Lucas, M-
dc.contributor.wosstandardWOS:Espinoza, E-
dc.contributor.wosstandardWOS:Arellano, JLP-
dc.contributor.wosstandardWOS:Gottstein, B-
dc.contributor.wosstandardWOS:Muro, A-
dc.date.coverdateMayo 2004en_US
dc.identifier.ulpgcen_US
dc.description.jcr2,545
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Estudio del proceso infeccioso y sus agentes causales-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Médicas y Quirúrgicas-
crisitem.author.orcid0000-0002-2936-8242-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNamePérez Arellano, José Luis-
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