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Title: Immunohistochemical distribution pattern of intermediate filament proteins and muscle actin in feline and human mammary carcinomas
Authors: Martín de las Mulas, J.
Espinosa De Los Monteros Y Zayas, Antonio 
Bautista, M. J.
Gómez Villamandos, J. C.
Morales, C.
UNESCO Clasification: 240111 Patología animal
320705 Patología comparativa
Keywords: Expression
Issue Date: 1994
Journal: Journal of Comparative Pathology 
Abstract: Thirty-seven feline and 38 human spontaneous mammary gland carcinomas were studied immunohistochemically. Commercially available antibodies directed against high and low molecular weight keratins (RCK-102 and NCL-5D3), vimentin, desmin, glial fibrillary acidic protein (GFAP), neurofilament (NF) proteins and muscle actin (HHF35) were used in the avidin biotin peroxidase complex (ABC) technique on formalin-fixed paraffin wax-embedded tumour tissue samples. Healthy feline and human mammary gland tissue adjacent to the neoplasms was also examined. The distribution pattern of intermediate filament proteins and muscle actin was comparable in healthy mammary gland tissue of the two species: both RCK-102 and NCL-5D3 antibodies reacted with luminal epithelial cells of ducts and acini, but basal/ myoepithelial cells were stained by RCK-102 exclusively. In addition, basal/ myoepithelial cells expressed vimentin and muscle actin in,both species, and GFAP was found in some feline basal/myoepithelial cells. No immunoreactivity to desmin and NF proteins;was observed. Feline mammary gland carcinoma cells reacted with RCK-102 (89%), NCL-5D3 (62%), vimentin (76%) and GFAP (30%) antibodies, while human mammary gland carcinoma cells reacted with RCK-102 (95%), NCL-5D3 (100%) and vimentin (13%) antibodies. HHF35 immunoreactivity was observed in stromal cells only. These results indicate that mammary gland carcinomas of both species share a heterogeneous immunophenotype with respect to intermediate filament proteins, which adds to the list of known similarities between mammary gland carcinomas of both species.
ISSN: 0021-9975
DOI: 10.1016/S0021-9975(05)80095-7
Source: Journal of Comparative Pathology [ISSN 0021-9975], v. 111 (4), p. 365-381, (Noviembre 1994)
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