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Title: A novel real-time PCR to detect Cetacean morbillivirus in Atlantic cetaceans
Authors: Groch, Kátia R.
Taniwaki, Sueli Akemi
Favero, Cíntia Maria
Brandão, Paulo Eduardo
Diaz Delgado, Josue 
Fernández Rodríguez, Antonio Jesús 
Catão-Dias, José Luiz
Sierra Pulpillo, Eva María 
UNESCO Clasification: 240119 Zoología marina
310907 Patología
Keywords: Cetacean Morbillivirus Diagnosis
Guiana Dolphin
Molecular Epidemiology
Phosphoprotein Gene
SYBR Green Real-Time RT-PCR
Issue Date: 2020
Journal: Journal of Virological Methods 
Abstract: Cetacean morbillivirus (CeMV, family Paramyxoviridae) is a re-emergent pathogen associated with severe epizootic outbreaks causing high mortality among cetaceans worldwide. Recently, CeMV caused an unusual mortality event of Guiana dolphins (Sotalia guianensis) in Brazil. Partial sequence of the viral phosphoprotein (P) gene showed that the Guiana dolphin morbillivirus (GDMV) might represent a new lineage of CeMV. This study aimed to develop a molecular technique to detect the most common CeMV strains known to circulate in the Atlantic Ocean: GDMV, Dolphin morbillivirus (DMV) and Pilot-whale morbillivirus (PWMV). A sensible real-time reverse transcription polymerase chain reaction (RT-qPCR) method based on intercalating dye, targeting the P gene was described. This assay successfully detected GDMV, PWMV and DMV from field samples. Its performance was compared to a RT-qPCR method that specifically detects GDMV. Both assays had high sensibility and excellent intra- and inter-assay reproducibility. A total of 109 field samples from 32 Guiana dolphins were screened for CeMV by conventional RT-PCR in parallel with the RT-qPCR assay. The detection rate increased from 32% to 60% by use of the novel RT-qPCR. The RT-qPCR assay described herein allows rapid and sensitive detection of Atlantic CeMV strains, and is potentially suitable for screening of CeMV globally.
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2020.113964
Source: Journal of Virological Methods [ISSN 0166-0934], v. 285, 113964, (Noviembre 2020)
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