Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/74507
Campo DC Valoridioma
dc.contributor.authorGroch, Kátia R.en_US
dc.contributor.authorTaniwaki, Sueli Akemien_US
dc.contributor.authorFavero, Cíntia Mariaen_US
dc.contributor.authorBrandão, Paulo Eduardoen_US
dc.contributor.authorDiaz Delgado, Josueen_US
dc.contributor.authorFernández Rodríguez, Antonio Jesúsen_US
dc.contributor.authorCatão-Dias, José Luizen_US
dc.contributor.authorSierra Pulpillo, Eva Maríaen_US
dc.date.accessioned2020-09-21T11:12:46Z-
dc.date.available2020-09-21T11:12:46Z-
dc.date.issued2020en_US
dc.identifier.issn0166-0934en_US
dc.identifier.otherScopus-
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/74507-
dc.description.abstractCetacean morbillivirus (CeMV, family Paramyxoviridae) is a re-emergent pathogen associated with severe epizootic outbreaks causing high mortality among cetaceans worldwide. Recently, CeMV caused an unusual mortality event of Guiana dolphins (Sotalia guianensis) in Brazil. Partial sequence of the viral phosphoprotein (P) gene showed that the Guiana dolphin morbillivirus (GDMV) might represent a new lineage of CeMV. This study aimed to develop a molecular technique to detect the most common CeMV strains known to circulate in the Atlantic Ocean: GDMV, Dolphin morbillivirus (DMV) and Pilot-whale morbillivirus (PWMV). A sensible real-time reverse transcription polymerase chain reaction (RT-qPCR) method based on intercalating dye, targeting the P gene was described. This assay successfully detected GDMV, PWMV and DMV from field samples. Its performance was compared to a RT-qPCR method that specifically detects GDMV. Both assays had high sensibility and excellent intra- and inter-assay reproducibility. A total of 109 field samples from 32 Guiana dolphins were screened for CeMV by conventional RT-PCR in parallel with the RT-qPCR assay. The detection rate increased from 32% to 60% by use of the novel RT-qPCR. The RT-qPCR assay described herein allows rapid and sensitive detection of Atlantic CeMV strains, and is potentially suitable for screening of CeMV globally.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Virological Methodsen_US
dc.sourceJournal of Virological Methods [ISSN 0166-0934], v. 285, 113964, (Noviembre 2020)en_US
dc.subject240119 Zoología marinaen_US
dc.subject310907 Patologíaen_US
dc.subject.otherCetacean Morbillivirus Diagnosisen_US
dc.subject.otherGuiana Dolphinen_US
dc.subject.otherMolecular Epidemiologyen_US
dc.subject.otherPhosphoprotein Geneen_US
dc.subject.otherSYBR Green Real-Time RT-PCRen_US
dc.titleA novel real-time PCR to detect Cetacean morbillivirus in Atlantic cetaceansen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jviromet.2020.113964en_US
dc.identifier.scopus85090403756-
dc.identifier.isi000577070700006-
dc.contributor.authorscopusid37072358500-
dc.contributor.authorscopusid23973987000-
dc.contributor.authorscopusid55313218300-
dc.contributor.authorscopusid55916312600-
dc.contributor.authorscopusid54787562800-
dc.contributor.authorscopusid56673009900-
dc.contributor.authorscopusid6602455809-
dc.contributor.authorscopusid15742962600-
dc.identifier.eissn1879-0984-
dc.relation.volume285en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid2038184-
dc.contributor.daisngid2162452-
dc.contributor.daisngid5527494-
dc.contributor.daisngid31533382-
dc.contributor.daisngid1111754-
dc.contributor.daisngid41646132-
dc.contributor.daisngid32226675-
dc.contributor.daisngid11943646-
dc.description.numberofpages6en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Groch, KR-
dc.contributor.wosstandardWOS:Taniwaki, SA-
dc.contributor.wosstandardWOS:Favero, CM-
dc.contributor.wosstandardWOS:Brandao, PE-
dc.contributor.wosstandardWOS:Diaz-Delgado, J-
dc.contributor.wosstandardWOS:Fernandez, A-
dc.contributor.wosstandardWOS:Catao-Dias, JL-
dc.contributor.wosstandardWOS:Sierra, E-
dc.date.coverdateNoviembre 2020en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr0,786
dc.description.jcr2,014
dc.description.sjrqQ3
dc.description.jcrqQ4
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0003-1216-5446-
crisitem.author.orcid0000-0001-5281-0521-
crisitem.author.orcid0000-0003-3749-8845-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameDiaz Delgado,Josue-
crisitem.author.fullNameFernández Rodríguez, Antonio Jesús-
crisitem.author.fullNameSierra Pulpillo, Eva María-
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