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Title: Early and Late Peritoneal and Hepatic Changes in Goats Immunized with Recombinant Cathepsin L1 and Infected with Fasciola hepatica
Authors: Zafra Leva,Rafael 
Perez-Ecija, R. A.
Buffoni, L.
Moreno, P.
Bautista, M. J.
Martinez-Moreno, A.
Mulcahy, G.
Dalton, J. P.
Pérez, J.
UNESCO Clasification: 240112 Parasitología animal
310907 Patología
Keywords: Cathepsin L1
Fasciola Hepatica
Immune Response
Issue Date: 2013
Journal: Journal of Comparative Pathology 
Abstract: The aim of the present study was to study peritoneal and hepatic changes during early [7-9 days postinfection (dpi)] and late [15 weeks postinfection (wpi)] infection of goats immunized with recombinant F. hepatica pro cathepsin L1 (rCL1) in Quil A and challenged with Fasciola hepatica. Despite finding no significant reduction in fluke burdens between the control and immunized group, at 15 dpi the rCL1-vaccinated group showed significantly higher weight gain and reduced severity of hepatic lesions compared with the control group that received only Quil A. In the rCL1-vaccinated group, two of three goats sacrificed at 7-9 dpi had little hepatic damage and had a higher percentage of peritoneal eosinophils and elevated induced nitric oxide synthase (iNOS) expression in peritoneal cells than the goats from the control group. Moreover, while these two goats showed a heavy infiltration of eosinophils surrounding migrating flukes, the remaining animals examined at 7-9 dpi had no inflammatory infiltration surrounding migrating flukes. Two out of seven goats in the rCL1-vaccinated group had low fluke burdens and little hepatic damage at 15 wpi, suggesting an effective protective response in some of the vaccinated goats. This protective response did not correlate with peripheral eosinophilia or with serum titres of anti-rCL1 immunoglobulin (Ig) G. The results of the present work suggest that an eosinophil-mediated immune response plays a crucial role in the early effective host response against F. hepatica in goats. Adjuvants designed to increase cell-mediated immunity should be tested in future vaccine trials against F. hepatica.
ISSN: 0021-9975
DOI: 10.1016/j.jcpa.2012.08.007
Source: Journal of Comparative Pathology [ISSN 0021-9975], v. 148 (4), p. 373-384, (Mayo 2013)
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