Please use this identifier to cite or link to this item:
Title: The relationship between the expression of fatty acyl desaturase 2 (Fads2) gene in peripheral blood cells (pbcs) and liver in gilthead seabream, sparus aurata broodstock fed a low n-3 lc-pufa diet
Authors: Shajahan, Ferosekhan 
Turkmen, Serhat
Xu, Hanlin 
Afonso, Juan Manuel 
Zamorano, Maria Jesus 
Kaushik, Sadasivam
Izquierdo, Marisol 
UNESCO Clasification: 251092 Acuicultura marina
Keywords: Biomarker
Fatty Acid
Fatty Acyl Desaturase (Fads2)
Lipid Metabolism
Issue Date: 2020
Project: Consumer driven Production: Integrating Innovative Approaches for Competitive and Sustainable Performance across the Mediterranean Aquaculture Value Chain 
Journal: Life 
Abstract: The principle aim of this study is to elucidate the relationship between the fatty acid desaturase 2 gene (fads2) expression pattern in peripheral blood cells (PBCs) and liver of gilthead seabream (GSB), Sparus aurata broodstock in order to determine the possible use of fads2 expression as a potential biomarker for the selection of broodstock. This selection could be utilized for breeding programs aiming to improve reproduction, health, and nutritional status. Passive Integrated Transponder (PIT)-tagged GSB broodstock (Male-1.22 ± 0.20 kg; 44.8 ± 2 cm and female-2.36 ± 0.64 kg; 55.1 cm) were fed a diet containing low levels of fish meal and fish oil (EPA 2.5; DHA 1.7 and n-3 LC-PUFA 4.6% TFA) for one month. After the feeding period, fads2 expression in PBCs and liver of both male and female broodstock were highly significantly correlated (r = 0.89; p < 0.001). Additionally, in male broodstock, liver fads2 expression was significantly correlated (p < 0.05) to liver contents in 16:0 (r = 0.95; p = 0.04) and total saturates (r = 0.97; p = 0.03) as well as to 20:3n–6/20:2n–6 (r = 0.98; p = 0.02) a Fads2 product/precursor ratio. Overall, we found a positive and significant correlation between fads2 expression levels in the PBCs and liver of GSB broodstock. PBCs fads2 expression levels indicate a strong potential for utilization as a non-invasive method to select animals having increased fatty acid bioconversion capability, better able to deal with diets free of fish meal and fish oil.
ISSN: 2075-1729
DOI: 10.3390/life10070117
Source: Life [EISSN 2075-1729], v. 10 (7), 117, p. 1-21, (Julio 2020)
Appears in Collections:Artículos
Adobe PDF (1,61 MB)
Show full item record

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.