|Title:||The relationship between the expression of fatty acyl desaturase 2 (Fads2) gene in peripheral blood cells (pbcs) and liver in gilthead seabream, sparus aurata broodstock fed a low n-3 lc-pufa diet||Authors:||Shajahan, Ferosekhan
Afonso, Juan Manuel
Zamorano, Maria Jesus
|UNESCO Clasification:||251092 Acuicultura marina||Keywords:||Biomarker
Fatty Acyl Desaturase (Fads2)
|Issue Date:||2020||Project:||Consumer driven Production: Integrating Innovative Approaches for Competitive and Sustainable Performance across the Mediterranean Aquaculture Value Chain||Journal:||Life||Abstract:||The principle aim of this study is to elucidate the relationship between the fatty acid desaturase 2 gene (fads2) expression pattern in peripheral blood cells (PBCs) and liver of gilthead seabream (GSB), Sparus aurata broodstock in order to determine the possible use of fads2 expression as a potential biomarker for the selection of broodstock. This selection could be utilized for breeding programs aiming to improve reproduction, health, and nutritional status. Passive Integrated Transponder (PIT)-tagged GSB broodstock (Male-1.22 ± 0.20 kg; 44.8 ± 2 cm and female-2.36 ± 0.64 kg; 55.1 cm) were fed a diet containing low levels of fish meal and fish oil (EPA 2.5; DHA 1.7 and n-3 LC-PUFA 4.6% TFA) for one month. After the feeding period, fads2 expression in PBCs and liver of both male and female broodstock were highly significantly correlated (r = 0.89; p < 0.001). Additionally, in male broodstock, liver fads2 expression was significantly correlated (p < 0.05) to liver contents in 16:0 (r = 0.95; p = 0.04) and total saturates (r = 0.97; p = 0.03) as well as to 20:3n–6/20:2n–6 (r = 0.98; p = 0.02) a Fads2 product/precursor ratio. Overall, we found a positive and significant correlation between fads2 expression levels in the PBCs and liver of GSB broodstock. PBCs fads2 expression levels indicate a strong potential for utilization as a non-invasive method to select animals having increased fatty acid bioconversion capability, better able to deal with diets free of fish meal and fish oil.||URI:||http://hdl.handle.net/10553/73923||ISSN:||2075-1729||DOI:||10.3390/life10070117||Source:||Life [EISSN 2075-1729], v. 10 (7), 117, p. 1-21, (Julio 2020)|
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