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http://hdl.handle.net/10553/54588
Título: | mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients | Autores/as: | Sieuwerts, Anieta M. Mostert, Bianca Bolt-De Vries, Joan Peeters, Dieter De Jongh, Felix E. Stouthard, Jacqueline M.L. Dirix, Luc Y. Van Dam, Peter A. Van Galen, Anne De Weerd, Vanja Kraan, Jaco Van Der Spoel, Petra Ramírez-Moreno, Raquel Van Deurzen, Carolien H.M. Smid, Marcel Yu, Jack X. Jiang, John Wang, Yixin Gratama, Jan W. Sleijfer, Stefan Foekens, John A. Martens, John W.M. |
Palabras clave: | Gene-Expression Molecular Markers Progression Biomarkers Prognosis, et al. |
Fecha de publicación: | 2011 | Editor/a: | 1078-0432 | Publicación seriada: | Clinical Cancer Research | Resumen: | Purpose: Molecular characterization of circulating tumor cells (CTC) holds great promise. Unfortunately, routinely isolated CTC fractions currently still contain contaminating leukocytes, which makes CTC-specific molecular characterization extremely challenging. In this study, we determined mRNA and microRNA (miRNA) expression of potentially CTC-specific genes that are considered to be clinically relevant in breast cancer.Experimental Design: CTCs were isolated with the epithelial cell adhesion molecule-based CellSearch Profile Kit. Selected genes were measured by real-time reverse transcriptase PCR in CTCs of 50 metastatic breast cancer patients collected before starting first-line systemic therapy in blood from 53 healthy blood donors (HBD) and in primary tumors of 8 of the patients. The molecular profiles were associated with CTC counts and clinical parameters and compared with the profiles generated from the corresponding primary tumors.Results: We identified 55 mRNAs and 10 miRNAs more abundantly expressed in samples from 32 patients with at least 5 CTCs in 7.5 mL of blood compared with samples from 9 patients without detectable CTCs and HBDs. Clustering analysis resulted in 4 different patient clusters characterized by 5 distinct gene clusters. Twice the number of patients from cluster 2 to 4 had developed both visceral and nonvisceral metastases. Comparing transcript levels in CTCs with those measured in corresponding primary tumors showed clinically relevant discrepancies in estrogen receptor and HER2 levels.Conclusions: Our study shows that molecular profiling of low numbers of CTCs in a high background of leukocytes is feasible and shows promise for further studies on the clinical relevance of molecular characterization of CTCs. Clin Cancer Res; 17(11); 3600-18. (C)2011 AACR. | URI: | http://hdl.handle.net/10553/54588 | ISSN: | 1078-0432 | DOI: | 10.1158/1078-0432.CCR-11-0255 | Fuente: | Clinical Cancer Research[ISSN 1078-0432],v. 17, p. 3600-3618 |
Colección: | Artículos |
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