Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54588
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dc.contributor.authorSieuwerts, Anieta M.
dc.contributor.authorMostert, Bianca
dc.contributor.authorBolt-De Vries, Joan
dc.contributor.authorPeeters, Dieter
dc.contributor.authorDe Jongh, Felix E.
dc.contributor.authorStouthard, Jacqueline M.L.
dc.contributor.authorDirix, Luc Y.
dc.contributor.authorVan Dam, Peter A.
dc.contributor.authorVan Galen, Anne
dc.contributor.authorDe Weerd, Vanja
dc.contributor.authorKraan, Jaco
dc.contributor.authorVan Der Spoel, Petra
dc.contributor.authorRamírez-Moreno, Raquel
dc.contributor.authorVan Deurzen, Carolien H.M.
dc.contributor.authorSmid, Marcel
dc.contributor.authorYu, Jack X.
dc.contributor.authorJiang, John
dc.contributor.authorWang, Yixin
dc.contributor.authorGratama, Jan W.
dc.contributor.authorSleijfer, Stefan
dc.contributor.authorFoekens, John A.
dc.contributor.authorMartens, John W.M.
dc.date.accessioned2019-02-18T11:47:35Z-
dc.date.available2019-02-18T11:47:35Z-
dc.date.issued2011
dc.identifier.issn1078-0432
dc.identifier.urihttp://hdl.handle.net/10553/54588-
dc.description.abstractPurpose: Molecular characterization of circulating tumor cells (CTC) holds great promise. Unfortunately, routinely isolated CTC fractions currently still contain contaminating leukocytes, which makes CTC-specific molecular characterization extremely challenging. In this study, we determined mRNA and microRNA (miRNA) expression of potentially CTC-specific genes that are considered to be clinically relevant in breast cancer.Experimental Design: CTCs were isolated with the epithelial cell adhesion molecule-based CellSearch Profile Kit. Selected genes were measured by real-time reverse transcriptase PCR in CTCs of 50 metastatic breast cancer patients collected before starting first-line systemic therapy in blood from 53 healthy blood donors (HBD) and in primary tumors of 8 of the patients. The molecular profiles were associated with CTC counts and clinical parameters and compared with the profiles generated from the corresponding primary tumors.Results: We identified 55 mRNAs and 10 miRNAs more abundantly expressed in samples from 32 patients with at least 5 CTCs in 7.5 mL of blood compared with samples from 9 patients without detectable CTCs and HBDs. Clustering analysis resulted in 4 different patient clusters characterized by 5 distinct gene clusters. Twice the number of patients from cluster 2 to 4 had developed both visceral and nonvisceral metastases. Comparing transcript levels in CTCs with those measured in corresponding primary tumors showed clinically relevant discrepancies in estrogen receptor and HER2 levels.Conclusions: Our study shows that molecular profiling of low numbers of CTCs in a high background of leukocytes is feasible and shows promise for further studies on the clinical relevance of molecular characterization of CTCs. Clin Cancer Res; 17(11); 3600-18. (C)2011 AACR.
dc.publisher1078-0432
dc.relation.ispartofClinical Cancer Research
dc.sourceClinical Cancer Research[ISSN 1078-0432],v. 17, p. 3600-3618
dc.subject.otherGene-Expression
dc.subject.otherMolecular Markers
dc.subject.otherProgression
dc.subject.otherBiomarkers
dc.subject.otherPrognosis
dc.subject.otherSurvival
dc.subject.otherTherapy
dc.subject.otherMirnas
dc.titlemRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients
dc.typeinfo:eu-repo/semantics/Article
dc.typeArticle
dc.identifier.doi10.1158/1078-0432.CCR-11-0255
dc.identifier.scopus79957894183
dc.identifier.isi000291200800012
dc.contributor.authorscopusid6602157921
dc.contributor.authorscopusid24169623900
dc.contributor.authorscopusid6603538971
dc.contributor.authorscopusid26653700700
dc.contributor.authorscopusid6603671317
dc.contributor.authorscopusid6602607364
dc.contributor.authorscopusid7005397716
dc.contributor.authorscopusid56481893600
dc.contributor.authorscopusid36648001000
dc.contributor.authorscopusid14017743600
dc.contributor.authorscopusid7003751962
dc.contributor.authorscopusid6504296163
dc.contributor.authorscopusid25959711200
dc.contributor.authorscopusid16067342400
dc.contributor.authorscopusid7006398514
dc.contributor.authorscopusid7405528333
dc.contributor.authorscopusid7404829199
dc.contributor.authorscopusid55734014800
dc.contributor.authorscopusid24733628000
dc.contributor.authorscopusid6601983607
dc.contributor.authorscopusid7006484454
dc.contributor.authorscopusid7201836882
dc.description.lastpage3618
dc.description.firstpage3600
dc.relation.volume17
dc.type2Artículo
dc.contributor.daisngid127351
dc.contributor.daisngid1279918
dc.contributor.daisngid1872513
dc.contributor.daisngid676821
dc.contributor.daisngid905162
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dc.contributor.daisngid1265474
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dc.contributor.daisngid3158692
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dc.contributor.daisngid7299455
dc.contributor.daisngid4038847
dc.contributor.daisngid19827496
dc.contributor.daisngid32888
dc.contributor.daisngid43725
dc.contributor.daisngid20416
dc.contributor.daisngid34295
dc.contributor.wosstandardWOS:Sieuwerts, AM
dc.contributor.wosstandardWOS:Mostert, B
dc.contributor.wosstandardWOS:Bolt-de Vries, J
dc.contributor.wosstandardWOS:Peeters, D
dc.contributor.wosstandardWOS:de Jongh, FE
dc.contributor.wosstandardWOS:Stouthard, JML
dc.contributor.wosstandardWOS:Dirix, LY
dc.contributor.wosstandardWOS:van Dam, PA
dc.contributor.wosstandardWOS:Van Galen, A
dc.contributor.wosstandardWOS:de Weerd, V
dc.contributor.wosstandardWOS:Kraan, J
dc.contributor.wosstandardWOS:van der Spoel, P
dc.contributor.wosstandardWOS:Ramirez-Moreno, R
dc.contributor.wosstandardWOS:van Deurzen, CHM
dc.contributor.wosstandardWOS:Smid, M
dc.contributor.wosstandardWOS:Yu, JX
dc.contributor.wosstandardWOS:Jiang, J
dc.contributor.wosstandardWOS:Wang, Y
dc.contributor.wosstandardWOS:Gratama, JW
dc.contributor.wosstandardWOS:Sleijfer, S
dc.contributor.wosstandardWOS:Foekens, JA
dc.contributor.wosstandardWOS:Martens, JWM
dc.date.coverdateJunio 2011
dc.identifier.ulpgces
dc.description.sjr4,4
dc.description.jcr7,742
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.fullNameRamírez Moreno, Raquel-
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