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Title: Application of microwave-assisted extraction and ultra-high performance liquid chromatography-tandem mass spectrometry for the analysis of sex hormones and corticosteroids in sewage sludge samples
Authors: Guedes-Alonso, Rayco 
Santana-Viera, Sergio 
Montesdeoca-Esponda, Sarah 
Afonso-Olivares, Cristina 
Sosa-Ferrera, Zoraida 
Santana-Rodríguez, José Juan 
UNESCO Clasification: 2302 Bioquímica
2391 Química ambiental
Keywords: Mass spectrometry
Microwave-assisted extraction
Steroid hormones
Ultra-high performance liquid chromatography
Issue Date: 2016
Publisher: 1618-2642
Project: Estrategias Para El Control y Remediación Natural de Compuestos Orgánicos Emergentes en Aguas Residuales. Impacto en El Medio Marino. 
Journal: Analytical and Bioanalytical Chemistry 
Abstract: Hormonal compounds are a concern to the international community because they can affect the aquatic biota and are therefore considered to be endocrine-disrupting compounds. These compounds have lipophilic properties, so they tend to accumulate in solid matrices, such as sewage sludge. This work presents the optimization of a microwave-assisted extraction process combined with ultra-high performance liquid chromatography–tandem mass spectrometry for the determination of 15 hormonal compounds in sludge samples. The proposed method has relative standard deviations below 23 %, good recoveries (over 71 %) for all compounds, detection limits that ranged from 1.1 to 7.9 ng g−1 and quantification limits which ranged from 3.7 to 26.3 ng g−1. The method was used to analyse sludge samples from four different wastewater treatment plants of Gran Canaria (Spain) with different wastewater treatments. 17β-estradiol, 17α-ethynylestradiol, norgestrel and cortisone were detected in sludge samples at concentrations that ranged from 17.3 to 1.44 × 103 ng g−1. The developed method permits the use of small quantities of sample and organic solvents, presents short extractions times and is the first one based on microwave-assisted extraction for the analysis of both sex hormones and corticosteroids.
ISSN: 1618-2642
DOI: 10.1007/s00216-016-9810-7
Source: Analytical and Bioanalytical Chemistry [ISSN 1618-2642], Septiembre 2016, v. 408 (24), p. 6833-6844
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