Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53521
Title: The androgen receptor does not mediate progestin regulation of progesterone biosynthesis in cultured rat granulosa cells
Authors: Quintana, J 
Fanjul, L. F. 
Estevez, F. 
Deniz Caceres, A. 
Marrero, I. 
Benitez, L.
Centol, I. 
Quintana, J.
Gonzalez, J.
Llanes, L.
Ruiz de Galarreta, C. M.
UNESCO Clasification: 32 Ciencias médicas
2403 Bioquímica
Keywords: Androgen receptor
Progestine
Progesterone
Issue Date: 1989
Journal: Biochemistry International 
Abstract: In the present investigation the influence of androgens and progestins on the FSH modulation of progesterone biosynthesis was studied in cultured rat granulosa cells. Cells obtained from the ovaries of immature estrogen treated rats were cultured for three days in serum free medium or in medium supplemented with FSH or CPA, with or without reduced androgen DHT or the synthetic progestin R5020 alone or in combination with the anti-androgen CPA. Treatment with FSH increased pregnenolone, progesterone and 20 alpha-OHP accumulation in the culture medium 20-, 14- and 7-fold, respectively. Furthermore FSH increased the activity of the enzyme 3 beta-HSD. Concurrent treatment with DHT or R5020 augmented the FSH stimulated steroidogenesis of cultured cells. The androgen enhancement of FSH stimulated steroidogenesis of cultured granulosa cells was blocked by concomitant treatment with CPA, whereas treatment of cultures with anti-androgen did not affect the stimulatory effect of the synthetic progestin R5020.
URI: http://hdl.handle.net/10553/45313
ISSN: 0158-5231
Source: Biochemistry International [ISSN 0158-5231], v. 19 (5), p. 977-984
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

2
checked on Jun 20, 2021

WEB OF SCIENCETM
Citations

2
checked on Jun 20, 2021

Page view(s)

25
checked on Jun 20, 2021

Google ScholarTM

Check


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.