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Title: Greater basal skeletal muscle AMPKα phosphorylation in men than in women: Associations with anaerobic performance
Authors: Torres-Peralta, Rafael 
Guadalupe Grau, Amelia 
Rodríguez-García, Lorena 
Morales Alamo, David 
Ponce-González, Jesús Gustavo
Pérez-Suarez, Ismael
Santana Rodríguez, Alfredo 
López Calbet, José Antonio 
UNESCO Clasification: 241106 Fisiología del ejercicio
2411 Fisiología humana
Keywords: AMPK
Anaerobic performance
Muscle phenotype
Muscle signalling
Issue Date: 2016
Publisher: 1746-1391
Journal: European Journal of Sport Science 
Abstract: Objectives: This study was designed to investigate the association of gender, fibre type composition, and anaerobic performance with the basal skeletal muscle signalling cascades regulating muscle phenotype. Design: Muscle biopsies were obtained from 25 men and 10 women all young and healthy. Methods. Protein phosphorylation of Thr172AMPKα, Ser221ACCβ, Thr286CaMKII as well as total protein abundance of PGC-1α, SIRT1, and CnA were measured by Western blot and anaerobic performance by the Wingate test. Results: Percent type I myosin heavy chain (MHC I) was lower in men (37.1 ± 10.4 vs. 58.5 ± 12.5, P <.01). Total, free testosterone and free androgen index were higher in men (11.5, 36.6 and 40.6 fold, respectively, P <.01). AMPKα phosphorylation was 2.2-fold higher in men compared to women (P <.01). Total Ser221ACCβ and Thr286CaMKII fractional phosphorylation tended to be higher in men (P =.1). PGC1-α and SIRT1 total protein expression was similar in men and women, whereas CnA tended to be higher in men (P =.1). Basal AMPKα phosphorylation was linearly related to the percentage of MHC I in men (r = 0.56; P <.01), but not in women. No association was observed between anaerobic performance and basal phosphorylations in men and women, analysed separately. Conclusion: In summary, skeletal muscle basal AMPKα phosphorylation is higher in men compared to women, with no apparent effect on anaerobic performance.
ISSN: 1746-1391
DOI: 10.1080/17461391.2015.1063701
Source: European Journal of Sport Science [ISSN 1746-1391], v. 16 (4), p. 455-464
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