Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/52252
Título: Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance
Autores/as: Estevez, M. Carmen
Belenguer, Jose
Gomez-Montes, Silvia
Miralles, Javier
Escuela, Alfonso M. 
Montoya, Angel 
Lechuga, Laura M.
Palabras clave: Natural-Water Samples
Pesticide-Residues
Liquid-Chromatography
Postharvest Fungicides
Environmental-Analysis, et al.
Fecha de publicación: 2012
Editor/a: 0003-2654
Publicación seriada: Analyst (London. 1877. Print) 
Resumen: A highly sensitive and specific SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ-protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 mu g L-1) and an IC50 of 3.2 nM (0.64 mu g L-1) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.
URI: http://hdl.handle.net/10553/52252
ISSN: 0003-2654
DOI: 10.1039/c2an36094b
Fuente: Analyst[ISSN 0003-2654],v. 137, p. 5659-5665
Colección:Artículos
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