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Title: Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance
Authors: Estevez, M. Carmen
Belenguer, Jose
Gomez-Montes, Silvia
Miralles, Javier
Escuela, Alfonso M. 
Montoya, Angel 
Lechuga, Laura M.
Keywords: Natural-Water Samples
Postharvest Fungicides
Environmental-Analysis, et al
Issue Date: 2012
Publisher: 0003-2654
Journal: Analyst (London. 1877. Print) 
Abstract: A highly sensitive and specific SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ-protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 mu g L-1) and an IC50 of 3.2 nM (0.64 mu g L-1) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.
ISSN: 0003-2654
DOI: 10.1039/c2an36094b
Source: Analyst[ISSN 0003-2654],v. 137, p. 5659-5665
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