Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52252
Title: Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance
Authors: Estevez, M. Carmen
Belenguer, Jose
Gomez-Montes, Silvia
Miralles, Javier
Escuela, Alfonso M. 
Montoya, Angel 
Lechuga, Laura M.
Keywords: Natural-Water Samples
Pesticide-Residues
Liquid-Chromatography
Postharvest Fungicides
Environmental-Analysis, et al
Issue Date: 2012
Publisher: 0003-2654
Journal: Analyst (London. 1877. Print) 
Abstract: A highly sensitive and specific SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ-protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 mu g L-1) and an IC50 of 3.2 nM (0.64 mu g L-1) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.
URI: http://hdl.handle.net/10553/52252
ISSN: 0003-2654
DOI: 10.1039/c2an36094b
Source: Analyst[ISSN 0003-2654],v. 137, p. 5659-5665
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

34
checked on May 16, 2021

WEB OF SCIENCETM
Citations

30
checked on May 16, 2021

Page view(s)

9
checked on May 17, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.