Please use this identifier to cite or link to this item:
Title: The effect of VLDL particles on the accuracy of a direct LDL-cholesterol method in type 2 diabetic patients
Authors: Wägner, Ana María 
Zapico, Edgar
Bonet, Rosa
Pérez, Antonio
Ordóñez-Llanos, Jordi
UNESCO Clasification: 32 Ciencias médicas
2403 Bioquímica
Keywords: LDL cholesterol
Direct method
Type 2 diabetes mellitus
Issue Date: 2003
Journal: Clinical biochemistry 
Abstract: Objective To assess the accuracy of the direct method LDL-c Plus, in type 2 diabetic patients. Methods LDL-c Plus was measured in 64 consecutive samples of type 2 diabetic patients and compared with betaquantification (BQ), Friedewald’s and an alternative formula. LDL-c Plus was also measured in the VLDL (d<1.006 Kg/L) fraction of these samples and in total serum and the VLDL fraction of a phenotype III patient, before and after diluting it with saline or VLDL from normolipidemic subjects. Results LDL-c Plus showed a significant, constant bias (-8.5 ± 5.6%) against BQ which correlated with VLDL-cholesterol/total triglyceride ratio (r = 0.760, p < 0.0005); bias decreased to zero when the ratio increased. In the VLDL fraction of the diabetic patients and the phenotype III patient LDL-c Plus measured 20.7 ± 11.6% and 56.2% of the cholesterol, respectively. Dilution with saline did not alter the latter percentage, whereas dilution with normolipidemic VLDL reduced it showing that LDL-c Plus recognized cholesterol-enriched particles in the d<1.006 Kg/L. Friedewald’s formula also showed a significant, constant bias (-3.1 ± 6.4%) against BQ, whereas the alternative formula did not (0.5 ± 6.1%). Both calculations classified patients better than LDL-c Plus did at NCEP cut-off points. Conclusions In type 2 diabetic patients, LDL-c Plus underestimates LDL-c but measures cholesterol associated to IDL particles in the d<1.006 Kg/L fraction. Although LDLc-Plus might be a better cardiovascular risk estimator when well standardized, at the moment, it does not seem to be superior to calculations.
ISSN: 0009-9120
DOI: 10.1016/S0009-9120(03)00006-7
Source: Clinical Biochemistry[ISSN 0009-9120],v. 36, p. 177-183
Appears in Collections:Artículos
Show full item record


checked on Mar 26, 2023

Page view(s)

checked on Mar 25, 2023

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.