Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51876
Title: Lipid accumulation in Schizochytrium G13/2S produced in continuous culture
Authors: Ganuza, E.
Izquierdo, M. S. 
UNESCO Clasification: 251092 Acuicultura marina
Keywords: Continuous culture
Docosahexaenoic acid
Lipid accumulation
Schizochytrium
Issue Date: 2007
Publisher: 0175-7598
Journal: Applied Microbiology and Biotechnology 
Abstract: Lipid and docosahexaenoic acid (DHA) accumulation into Schizochytrium G13/2S was studied under batch and continuous culture. Different glucose and glutamate concentrations were supplemented in a defined medium. During batch cultivation, lipid accumulation, 35% total fatty acids (TFA) occurred at the arithmetic growth phase but ceased when cell growth stopped. When continuous culture was performed under different glutamate concentrations, nitrogen-growth-limiting conditions induced the accumulation of 30–28% TFA in Schizochytrium. As the dilution rate decreased from 0.08 to 0.02 h−1, both cell dry weight and TFA content of the cell increased. Under a constant dilution rate of 0.04 h−1, carbon-limiting conditions decreased the TFA to 22%. Fatty acid profile was not affected by the different nutrient concentrations provided during continuous culture. Consequently, lipid accumulation can be induced through the carbon and nitrogen source concentration in the medium to maximise the TFA and subsequently DHA productivity by this microorganism.
URI: http://hdl.handle.net/10553/51876
ISSN: 0175-7598
DOI: 10.1007/s00253-007-1019-4
Source: Applied Microbiology and Biotechnology [ISSN 0175-7598], v. 76, p. 985-990
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

64
checked on Jul 25, 2021

WEB OF SCIENCETM
Citations

51
checked on May 30, 2021

Page view(s)

43
checked on Jun 22, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.