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Title: Cryopreservation of semen in the dog: Use of ultra-freezers of -152°C as a viable alternative to liquid nitrogen
Authors: Álamo, Desirée
Batista, Miguel 
González, Fernando
Rodríguez, Noemí
Cruz, Guadalupe
Cabrera, Fernando 
Gracia, Anselmo 
Keywords: Canine Spermatozoa
Intrauterine Insemination
Postthaw Survival
3 Extenders
Frozen, et al
Issue Date: 2005
Publisher: 0093-691X
Journal: Theriogenology 
Abstract: This experimental work was carried out to validate the use of a -152 degrees C ultra-low temperature freezer to freeze and store canine semen. The semen of three dogs was pooled and processed to obtain a final dilution with a concentration of 100 x 10(6) spermatozoa/mL, glycerol at 5% and Equex at 0.5%. Then, four freezing protocols were tested to evaluate the cryosurvival of sperm at 1, 7, 30, 60 and 120 days after freezing: (I) semen was frozen and stored in liquid nitrogen; (11) semen was frozen in liquid nitrogen and stored in the ultra-low freezer at -152 degrees C; (III) semen was frozen in the vapour of liquid nitrogen and stored in the ultra-low freezer at -152 degrees C; (IV) semen was frozen and stored in the ultra-low freezer at -152 degrees C. Data were statistically analyzed by repeated measures analysis of variance to determine the effect of the freezing protocol and time on the sperm characteristics assessed. The percentages of sperm motility and of dead/live spermatozoa were similar throughout the experimental period, with no significant differences (P < 0.05) to be observed between four different freezing techniques tested. At 120 days after freezing, the percentage of abnormal cells and the percentage of sperm cells with abnormal acrosome were not significantly different between the freezing techniques. Although the number of dogs used was slightly low, in vitro results of this preliminary study showed that the use of ultra-freezers at -152 degrees C to freeze and store canine semen could be a viable alternative to liquid nitrogen. (c) 2004 Elsevier Inc. All rights reserved.
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2004.03.016
Source: Theriogenology[ISSN 0093-691X],v. 63, p. 72-82
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