Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51547
Título: Influence of Cool Storage before Freezing on the Quality of Frozen-Thawed Semen Samples in Dogs
Autores/as: Santana, M.
Batista Arteaga, Miguel 
Alamo, D.
González, F.
Niño, T.
Cabrera Martín, Fernando 
Gracia Molina, Anselmo 
Clasificación UNESCO: 310411 Reproducción
Palabras clave: Semen quality
Frozen-thawed samples
Dogs
Fecha de publicación: 2013
Publicación seriada: Reproduction in Domestic Animals 
Resumen: The aim of this study was to determinate the semen quality of frozen–thawed samples that were chilled for up to 2 days before freezing. The ejaculates (n = 18) from six dogs were collected, pooled and divided into six aliquots. The first aliquot (C, control) was frozen in liquid nitrogen using a conventional protocol to reach a final concentration of 100 × 106 spermatozoa/ml, 20% egg yolk and 5% glycerol. The remaining five aliquots were diluted with a chilled extender (Tris‐glucose and 20% egg yolk) and cooled at 4°C as follows: R1, the semen was cooled for 1 h; R6, the semen was cooled for 6 h; R12, the semen was cooled for 12 h; R24, the semen was cooled for 24 h and R48, the semen was cooled for 48 h. After the chilling period, a second extender was added (Tris‐glucose, 20% egg yolk, 10% glycerol and Equex at 1%) to reach a final composition similar to aliquot C, and then, the semen samples (R1, R6, R12, R24 and R48) were frozen in liquid nitrogen. The post‐thaw sperm quality was assessed in 30 straws from each experimental group. After freezing–thawing, the total sperm motility (approximately 60–70%) in the semen chilled for up to 48 h did not show any differences from the samples frozen by the conventional cryopreservation method (63.2%). No significant differences were detected in the percentages of abnormal sperm cells among the fresh semen, the control group and the frozen samples after the different cooling times. Finally, the post‐thaw percentages of damaged acrosomes showed a very uniform distribution, with mean values ranging between 7% and 10.5%. The results clearly demonstrated that cooling the semen up to 48 h before freezing did not produce a decrease in the semen quality when was compared with semen frozen by a traditional procedure.
URI: http://hdl.handle.net/10553/51547
ISSN: 0936-6768
DOI: 10.1111/j.1439-0531.2012.02124.x
Fuente: Reproduction in Domestic Animals [ISSN 0936-6768], v. 48 (1), p. 165-170
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