Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51547
DC FieldValueLanguage
dc.contributor.authorSantana, M.en_US
dc.contributor.authorBatista Arteaga, Miguelen_US
dc.contributor.authorAlamo, D.en_US
dc.contributor.authorGonzález, F.en_US
dc.contributor.authorNiño, T.en_US
dc.contributor.authorCabrera Martín, Fernandoen_US
dc.contributor.authorGracia Molina, Anselmoen_US
dc.date.accessioned2018-11-25T01:35:07Z-
dc.date.available2018-11-25T01:35:07Z-
dc.date.issued2013en_US
dc.identifier.issn0936-6768en_US
dc.identifier.urihttp://hdl.handle.net/10553/51547-
dc.description.abstractThe aim of this study was to determinate the semen quality of frozen–thawed samples that were chilled for up to 2 days before freezing. The ejaculates (n = 18) from six dogs were collected, pooled and divided into six aliquots. The first aliquot (C, control) was frozen in liquid nitrogen using a conventional protocol to reach a final concentration of 100 × 106 spermatozoa/ml, 20% egg yolk and 5% glycerol. The remaining five aliquots were diluted with a chilled extender (Tris‐glucose and 20% egg yolk) and cooled at 4°C as follows: R1, the semen was cooled for 1 h; R6, the semen was cooled for 6 h; R12, the semen was cooled for 12 h; R24, the semen was cooled for 24 h and R48, the semen was cooled for 48 h. After the chilling period, a second extender was added (Tris‐glucose, 20% egg yolk, 10% glycerol and Equex at 1%) to reach a final composition similar to aliquot C, and then, the semen samples (R1, R6, R12, R24 and R48) were frozen in liquid nitrogen. The post‐thaw sperm quality was assessed in 30 straws from each experimental group. After freezing–thawing, the total sperm motility (approximately 60–70%) in the semen chilled for up to 48 h did not show any differences from the samples frozen by the conventional cryopreservation method (63.2%). No significant differences were detected in the percentages of abnormal sperm cells among the fresh semen, the control group and the frozen samples after the different cooling times. Finally, the post‐thaw percentages of damaged acrosomes showed a very uniform distribution, with mean values ranging between 7% and 10.5%. The results clearly demonstrated that cooling the semen up to 48 h before freezing did not produce a decrease in the semen quality when was compared with semen frozen by a traditional procedure.en_US
dc.languageengen_US
dc.relation.ispartofReproduction in Domestic Animalsen_US
dc.sourceReproduction in Domestic Animals [ISSN 0936-6768], v. 48 (1), p. 165-170en_US
dc.subject310411 Reproducciónen_US
dc.subject.otherSemen qualityen_US
dc.subject.otherFrozen-thawed samplesen_US
dc.subject.otherDogsen_US
dc.titleInfluence of Cool Storage before Freezing on the Quality of Frozen-Thawed Semen Samples in Dogsen_US
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1111/j.1439-0531.2012.02124.x
dc.identifier.scopus84872496519-
dc.identifier.isi000313875800029
dc.contributor.authorscopusid35936129800-
dc.contributor.authorscopusid7005481929-
dc.contributor.authorscopusid57212844863
dc.contributor.authorscopusid6507009446-
dc.contributor.authorscopusid57194243767-
dc.contributor.authorscopusid23091540700-
dc.contributor.authorscopusid7006639324-
dc.contributor.authorscopusid7006185082-
dc.description.lastpage170-
dc.description.firstpage165-
dc.relation.volume48-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoes
dc.contributor.daisngid746940
dc.contributor.daisngid30341669
dc.contributor.daisngid1791005
dc.contributor.daisngid3001453
dc.contributor.daisngid2567189
dc.contributor.daisngid2128761
dc.contributor.daisngid9536463
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Santana, M
dc.contributor.wosstandardWOS:Batista, M
dc.contributor.wosstandardWOS:Alamo, D
dc.contributor.wosstandardWOS:Gonzalez, F
dc.contributor.wosstandardWOS:Nino, T
dc.contributor.wosstandardWOS:Cabrera, F
dc.contributor.wosstandardWOS:Gracia, A
dc.date.coverdateFebrero 2013
dc.identifier.ulpgces
dc.description.sjr0,66
dc.description.jcr1,177
dc.description.sjrqQ2
dc.description.jcrqQ2
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptMedicina Veterinaria e Investigación Terapeútica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptReproducción Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0001-9753-4786-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameBatista Arteaga, Miguel-
crisitem.author.fullNameCabrera Martín, Fernando-
crisitem.author.fullNameGracia Molina, Anselmo-
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