Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51162
Title: Telmisartan-induced eNOS gene expression is partially independent of its PPAR-gamma agonist property
Authors: Ríos, Nisa Buset
Rodriguez Esparragon, Francisco 
Rodriguez Perez, Jose C. 
Keywords: Activated-Receptor-Gamma
Nitric-Oxide Synthase
Human Adipose-Tissue
3T3-L1 Adipocytes
Modulating Activity, et al
Issue Date: 2012
Publisher: 0147-958X
Journal: Clinical and Investigative Medicine 
Abstract: Purpose: Telmisartan, an angiotensin II receptor blocker (ARB), also acts as an activator of peroxisome proliferator-activated receptor-gamma (PPAR-gamma; PPAR-gamma). Several studies have explored the PPAR-gamma-endothelial nitric oxide synthase (eNOS) pathway associated with improvement of endothelial function by telmisartan. The ability of telmisartan to induce gene expression and protein level of eNOS and PPAR gamma in adipocytes was investigated.Methods: Expression of aP2, PPAR gamma, eNOS and iNOS genes were measured using the quantitative real-time polymerase chain reaction. The changes, at the protein level, were explored by Western blot, which evaluated the native and phosphorylated eNOS forms, eNOS-Ser(1177) and eNOS-Thr(495).Results: Adipocytes, exposed to telmisartan, exhibited an increase in PPAR gamma gene expression but a decrease in protein level. Nonetheless, after the exposure to telmisartan, eNOS-Ser(1177) phosphorylation, associated with eNOS activity increment, reached its highest value while eNOS-Thr(495) phosphorylation, involved in the inhibition of eNOS activity, showed its lowest value.Conclusion: The results suggest that telmisartan preserves eNOS activity via a mechanism that is partially independent of the PPAR gamma-eNOS pathway in adipocytes.
URI: http://hdl.handle.net/10553/51162
ISSN: 0147-958X
Source: Clinical And Investigative Medicine[ISSN 0147-958X],v. 35 (2), p. E55-E64
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