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Title: Bed rest reduces metabolic protein content and abolishes exercise-induced mRNA responses in human skeletal muscle
Authors: Ringholm, Stine
Bienso, Rasmus S.
Kiilerich, Kristian
Guadalupe-Grau, Amelia
Aachmann-Andersen, Niels Jacob
Saltin, Bengt
Plomgaard, Peter
Lundby, Carsten
Wojtaszewski, Jorgen F.P.
Calbet, Jose A. 
Pilegaard, Henriette
UNESCO Clasification: 241106 Fisiología del ejercicio
Keywords: physical inactivity
oxidative capacity
peroxisome proliferator-activated receptor- coactivator-1
Issue Date: 2011
Publisher: 0193-1849
Journal: American Journal of Physiology - Endocrinology and Metabolism 
Abstract: The aim was to test the hypothesis that 7 days of bed rest reduces mitochondrial number and expression and activity of oxidative proteins in human skeletal muscle but that exercise-induced intracellular signaling as well as mRNA and microRNA (miR) responses are maintained after bed rest. Twelve young, healthy male subjects completed 7 days of bed rest with vastus lateralis muscle biopsies taken before and after bed rest. In addition, muscle biopsies were obtained from six of the subjects prior to, immediately after, and 3 h after 45 min of one-legged knee extensor exercise performed before and after bed rest. Maximal oxygen uptake decreased by 4%, and exercise endurance decreased nonsignificantly, by 11%, by bed rest. Bed rest reduced skeletal muscle mitochondrial DNA/nuclear DNA content 15%, hexokinase II and sirtuin 1 protein content 45%, 3-hydroxyacyl-CoA dehydrogenase and citrate synthase activity 8%, and miR-1 and miR-133a content 10%. However, cytochrome c and vascular endothelial growth factor (VEGF) protein content as well as capillarization did not change significantly with bed rest. Acute exercise increased AMP-activated protein kinase phosphorylation, peroxisome proliferator activated receptor- coactivator-1, and VEGF mRNA content in skeletal muscle before bed rest, but the responses were abolished after bed rest. The present findings indicate that only 7 days of physical inactivity reduces skeletal muscle metabolic capacity as well as abolishes exercise-induced adaptive gene responses, likely reflecting an interference with the ability of skeletal muscle to adapt to exercise.
ISSN: 0193-1849
DOI: 10.1152/ajpendo.00230.2011
Source: American Journal Of Physiology-Endocrinology And Metabolism[ISSN 0193-1849],v. 301 (4), p. E649-E658
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