Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/50941
Título: Bed rest reduces metabolic protein content and abolishes exercise-induced mRNA responses in human skeletal muscle
Autores/as: Ringholm, Stine
Bienso, Rasmus S.
Kiilerich, Kristian
Guadalupe-Grau, Amelia
Aachmann-Andersen, Niels Jacob
Saltin, Bengt
Plomgaard, Peter
Lundby, Carsten
Wojtaszewski, Jorgen F.P.
Calbet, Jose A. 
Pilegaard, Henriette
Clasificación UNESCO: 241106 Fisiología del ejercicio
Palabras clave: physical inactivity
oxidative capacity
peroxisome proliferator-activated receptor- coactivator-1
capillarization
Fecha de publicación: 2011
Editor/a: 0193-1849
Publicación seriada: American Journal of Physiology - Endocrinology and Metabolism 
Resumen: The aim was to test the hypothesis that 7 days of bed rest reduces mitochondrial number and expression and activity of oxidative proteins in human skeletal muscle but that exercise-induced intracellular signaling as well as mRNA and microRNA (miR) responses are maintained after bed rest. Twelve young, healthy male subjects completed 7 days of bed rest with vastus lateralis muscle biopsies taken before and after bed rest. In addition, muscle biopsies were obtained from six of the subjects prior to, immediately after, and 3 h after 45 min of one-legged knee extensor exercise performed before and after bed rest. Maximal oxygen uptake decreased by 4%, and exercise endurance decreased nonsignificantly, by 11%, by bed rest. Bed rest reduced skeletal muscle mitochondrial DNA/nuclear DNA content 15%, hexokinase II and sirtuin 1 protein content 45%, 3-hydroxyacyl-CoA dehydrogenase and citrate synthase activity 8%, and miR-1 and miR-133a content 10%. However, cytochrome c and vascular endothelial growth factor (VEGF) protein content as well as capillarization did not change significantly with bed rest. Acute exercise increased AMP-activated protein kinase phosphorylation, peroxisome proliferator activated receptor- coactivator-1, and VEGF mRNA content in skeletal muscle before bed rest, but the responses were abolished after bed rest. The present findings indicate that only 7 days of physical inactivity reduces skeletal muscle metabolic capacity as well as abolishes exercise-induced adaptive gene responses, likely reflecting an interference with the ability of skeletal muscle to adapt to exercise.
URI: http://hdl.handle.net/10553/50941
ISSN: 0193-1849
DOI: 10.1152/ajpendo.00230.2011
Fuente: American Journal Of Physiology-Endocrinology And Metabolism[ISSN 0193-1849],v. 301 (4), p. E649-E658
Colección:Artículos
Vista completa

Google ScholarTM

Verifica

Altmetric


Comparte



Exporta metadatos



Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.