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Title: Ayanin diacetate-induced cell death is amplified by TRAIL in human leukemia cells
Authors: Marrero, María Teresa
Estévez, Sara
Negrín, Gledy
Quintana, Jose 
López, Mariana
Pérez, Francisco J. 
Triana, Jorge
León, Francisco
Estévez, Francisco 
Keywords: Clinical-Implications
Induced Apoptosis
Targeting Death
Decoy Receptors
Cancer, et al
Issue Date: 2012
Publisher: 0006-291X
Project: Evaluación de Potenciales Compuestos Antileucémicos. 
Journal: Biochemical and biophysical research communications (Print) 
Abstract: Here we demonstrate that the semi-synthetic flavonoid ayanin diacetate induces cell death selectively in leukemia cells without affecting the proliferation of normal lymphocytes. Incubation of human leukemia cells with ayanin diacetate induced G(2)-M phase cell cycle arrest and apoptosis which was prevented by the non-specific caspase inhibitor z-VAD-fmk and reduced by the overexpression of Bcl-x(L). Ayanin diacetate-induced cell death was found to be associated with: (i) loss of inner mitochondrial membrane potential, (ii) the release of cytochrome c, (iii) the activation of multiple caspases, (iv) cleavage of poly(ADP-ribose) polymerase and (v) the up-regulation of death receptors for TRAIL, DR4 and DR5. Moreover, the combined treatment with ayanin diacetate and TRAIL amplified cell death, compared to single treatments. These results provide a basis for further exploring the potential applications of this combination for the treatment of cancer. (C) 2012 Elsevier Inc. All rights reserved.
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2012.10.017
Source: Biochemical and Biophysical Research Communications[ISSN 0006-291X],v. 428, p. 116-120
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