|Title:||Ayanin diacetate-induced cell death is amplified by TRAIL in human leukemia cells||Authors:||Marrero, María Teresa
Pérez, Francisco J.
|UNESCO Clasification:||32 Ciencias médicas
Cancer, et al
|Issue Date:||2012||Project:||Evaluación de Potenciales Compuestos Antileucémicos.||Journal:||Biochemical and biophysical research communications (Print)||Abstract:||Here we demonstrate that the semi-synthetic flavonoid ayanin diacetate induces cell death selectively in leukemia cells without affecting the proliferation of normal lymphocytes. Incubation of human leukemia cells with ayanin diacetate induced G(2)-M phase cell cycle arrest and apoptosis which was prevented by the non-specific caspase inhibitor z-VAD-fmk and reduced by the overexpression of Bcl-x(L). Ayanin diacetate-induced cell death was found to be associated with: (i) loss of inner mitochondrial membrane potential, (ii) the release of cytochrome c, (iii) the activation of multiple caspases, (iv) cleavage of poly(ADP-ribose) polymerase and (v) the up-regulation of death receptors for TRAIL, DR4 and DR5. Moreover, the combined treatment with ayanin diacetate and TRAIL amplified cell death, compared to single treatments. These results provide a basis for further exploring the potential applications of this combination for the treatment of cancer.||URI:||http://hdl.handle.net/10553/50549||ISSN:||0006-291X||DOI:||10.1016/j.bbrc.2012.10.017||Source:||Biochemical and Biophysical Research Communications[ISSN 0006-291X],v. 428(1), p. 116-120 (Noviembre 2012)|
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