|Title:||Class I chitinases with hevein-like domain, but not class II enzymes, are relevant chestnut and avocado allergens||Authors:||Diaz-Perales, Araceli
|UNESCO Clasification:||32 Ciencias médicas
|Issue Date:||1998||Journal:||Journal of Allergy and Clinical Immunology||Abstract:||Background: Several foods associated with the latex-fruit syndrome present relevant allergens of around 30 kd. Neither these components nor any other responsible for the reported cross-reactions have been identified and purified. Objective: We sought to isolate and characterize the 30 kd allergens from avocado fruit and chestnut seed, two of the main allergenic foods linked with latex allergy. Methods: Sera from patients allergic to chestnut and avocado were selected according to clinical symptoms, specific IgE levels, and positive skin prick test responses. Class I and II chitinases were purified by affinity and cation-exchange chromatography and characterized by specific IgE and anti-chitinase immunodetection, immunoblot inhibition assays, enzymatic activity tests, and N-terminal sequencing. Results: Relevant 32 kd allergens were detected by specific IgE immunodetection in both avocado and chestnut crude extracts. The same bands, together with others of 25 kd, were revealed by a monospecific antiserum against class II chitinases. Purification and characterization of the 32 kd allergens from both plant sources allowed their identification as class I chitinases with an N-terminal hevein-domain. The purified allergens fully inhibited IgE binding by the corresponding crude extract when tested in immunoblot inhibition assays. Highly related 25 kd class II chitinases that lack the hevein-like domain were also isolated from the same protein preparations. No IgE-binding capacity was shown by these class II enzymes. Conclusion: Class I chitinases are relevant allergens of avocado and chestnut and could be the panallergens responsible for the latex-fruit syndrome. The hevein-like domain seems to be involved in their allergenic reactivity.||URI:||http://hdl.handle.net/10553/50255||ISSN:||0091-6749||DOI:||10.1016/S0091-6749(98)70063-6||Source:||Journal of Allergy and Clinical Immunology[ISSN 0091-6749],v. 102, p. 127-133|
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