Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/50255
Campo DC Valoridioma
dc.contributor.authorDiaz-Perales, Aracelien_US
dc.contributor.authorCollada, Carmenen_US
dc.contributor.authorBlanco, Carlosen_US
dc.contributor.authorSánchez-Monge, Rosaen_US
dc.contributor.authorCarrillo, Teresaen_US
dc.contributor.authorAragoncillo, Ciprianoen_US
dc.contributor.authorSalcedo, Gabrielen_US
dc.date.accessioned2018-11-24T14:37:01Z-
dc.date.available2018-11-24T14:37:01Z-
dc.date.issued1998en_US
dc.identifier.issn0091-6749en_US
dc.identifier.urihttp://hdl.handle.net/10553/50255-
dc.description.abstractBackground: Several foods associated with the latex-fruit syndrome present relevant allergens of around 30 kd. Neither these components nor any other responsible for the reported cross-reactions have been identified and purified. Objective: We sought to isolate and characterize the 30 kd allergens from avocado fruit and chestnut seed, two of the main allergenic foods linked with latex allergy. Methods: Sera from patients allergic to chestnut and avocado were selected according to clinical symptoms, specific IgE levels, and positive skin prick test responses. Class I and II chitinases were purified by affinity and cation-exchange chromatography and characterized by specific IgE and anti-chitinase immunodetection, immunoblot inhibition assays, enzymatic activity tests, and N-terminal sequencing. Results: Relevant 32 kd allergens were detected by specific IgE immunodetection in both avocado and chestnut crude extracts. The same bands, together with others of 25 kd, were revealed by a monospecific antiserum against class II chitinases. Purification and characterization of the 32 kd allergens from both plant sources allowed their identification as class I chitinases with an N-terminal hevein-domain. The purified allergens fully inhibited IgE binding by the corresponding crude extract when tested in immunoblot inhibition assays. Highly related 25 kd class II chitinases that lack the hevein-like domain were also isolated from the same protein preparations. No IgE-binding capacity was shown by these class II enzymes. Conclusion: Class I chitinases are relevant allergens of avocado and chestnut and could be the panallergens responsible for the latex-fruit syndrome. The hevein-like domain seems to be involved in their allergenic reactivity.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Allergy and Clinical Immunologyen_US
dc.sourceJournal of Allergy and Clinical Immunology[ISSN 0091-6749],v. 102, p. 127-133en_US
dc.subject32 Ciencias médicasen_US
dc.subject320701 Alergiasen_US
dc.subject.otherChitinasesen_US
dc.subject.otherFood Hypersensitivityen_US
dc.subject.otherImmunoglobulin Een_US
dc.subject.otherLauraceaeen_US
dc.subject.otherLectinsen_US
dc.titleClass I chitinases with hevein-like domain, but not class II enzymes, are relevant chestnut and avocado allergensen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/S0091-6749(98)70063-6en_US
dc.identifier.scopus0031817192-
dc.contributor.authorscopusid6602872247-
dc.contributor.authorscopusid6603435995-
dc.contributor.authorscopusid57189070218-
dc.contributor.authorscopusid7003304866-
dc.contributor.authorscopusid7003526269-
dc.contributor.authorscopusid6701421244-
dc.contributor.authorscopusid7006342147-
dc.description.lastpage133en_US
dc.description.firstpage127en_US
dc.relation.volume102en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages7en_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr4,509-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Patología médica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Médicas y Quirúrgicas-
crisitem.author.orcid0000-0002-3047-8908-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameCarrillo Díaz, Teresa-
Colección:Artículos
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