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http://hdl.handle.net/10553/50002
Title: | A Simplified Method for the Preparation of Transcriptionally Active Liver Nuclear Extracts | Authors: | Hattori, Masahira Tugores, Antonio Veloz, Linda Brenner, David A. Karin, Michael |
UNESCO Clasification: | 32 Ciencias médicas 320102 Genética clínica 2407 Biología celular |
Keywords: | Nuclear extracts Plasmid DNA DNA binding proteins |
Issue Date: | 1990 | Journal: | DNA and Cell Biology | Abstract: | We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts. | URI: | http://hdl.handle.net/10553/50002 | ISSN: | 1044-5498 | DOI: | 10.1089/dna.1990.9.777 | Source: | DNA and Cell Biology[ISSN 1044-5498],v. 9(10), p. 777-781 (Diciembre 1990) |
Appears in Collections: | Artículos |
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