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Title: A Simplified Method for the Preparation of Transcriptionally Active Liver Nuclear Extracts
Authors: Hattori, Masahira
Tugores, Antonio 
Veloz, Linda
Brenner, David A.
Karin, Michael
UNESCO Clasification: 32 Ciencias médicas
320102 Genética clínica
2407 Biología celular
Keywords: Nuclear extracts
Plasmid DNA
DNA binding proteins
Issue Date: 1990
Journal: DNA and Cell Biology 
Abstract: We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.
ISSN: 1044-5498
DOI: 10.1089/dna.1990.9.777
Source: DNA and Cell Biology[ISSN 1044-5498],v. 9(10), p. 777-781 (Diciembre 1990)
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