Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/49903
Title: Modification of the electron transport system (ETS) method for routine measurements of respiratory rates of zooplankton
Authors: Gomez, M 
Torres Curbelo, Santiago 
Hernzández-León, S. 
UNESCO Clasification: 251001 Oceanografía biológica
Keywords: Zooplankton
Respiratory Electron Transport System activity (ETS)
Issue Date: 1996
Publisher: 0257-7615
Journal: South African Journal of Marine Science 
Abstract: Respiratory Electron Transport System activity (ETS) was determined in cell-free homogenates of mesozooplankton samples. Several assays were run to investigate possible improvements to the ETS method and to reduce sample processing time. The results showed that: (i) liquid nitrogen is a suitable method for preserving ETS activity for up to at least two months; (ii) the processed sample can be stored for up to 90 minutes in an ice-water bath without loss of activity; (iii) a glass-fibre filter, normally used in phytoplankton assays, is not necessary to achieve efficient enzyme extraction; (iv) centrifugation can be done prior to the assay without any significant loss in activity; and (v) the partitioned enzyme activities were different from the overall ETS activity, with NADH- dehydrogenase activity playing the major role (71 %) and succinate dehydrogenase the minor (<5%). The overall precision of the methods was about 10%, and automation of the method is suggested as a means of standardizing the precision for routine use at sea.
URI: http://hdl.handle.net/10553/49903
ISSN: 0257-7615
DOI: 10.2989/025776196784158446
Source: South African Journal of Marine Science [ISSN 0257-7615], v. 17, p. 15-20
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