Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/49903
DC FieldValueLanguage
dc.contributor.authorGomez, Men_US
dc.contributor.authorTorres Curbelo, Santiagoen_US
dc.contributor.authorHernzández-León, S.en_US
dc.contributor.otherGomez, May-
dc.date.accessioned2018-11-24T11:39:46Z-
dc.date.available2018-11-24T11:39:46Z-
dc.date.issued1996en_US
dc.identifier.issn0257-7615en_US
dc.identifier.urihttp://hdl.handle.net/10553/49903-
dc.description.abstractRespiratory Electron Transport System activity (ETS) was determined in cell-free homogenates of mesozooplankton samples. Several assays were run to investigate possible improvements to the ETS method and to reduce sample processing time. The results showed that: (i) liquid nitrogen is a suitable method for preserving ETS activity for up to at least two months; (ii) the processed sample can be stored for up to 90 minutes in an ice-water bath without loss of activity; (iii) a glass-fibre filter, normally used in phytoplankton assays, is not necessary to achieve efficient enzyme extraction; (iv) centrifugation can be done prior to the assay without any significant loss in activity; and (v) the partitioned enzyme activities were different from the overall ETS activity, with NADH- dehydrogenase activity playing the major role (71 %) and succinate dehydrogenase the minor (<5%). The overall precision of the methods was about 10%, and automation of the method is suggested as a means of standardizing the precision for routine use at sea.en_US
dc.languageengen_US
dc.publisher0257-7615-
dc.relation.ispartofSouth African Journal of Marine Scienceen_US
dc.sourceSouth African Journal of Marine Science [ISSN 0257-7615], v. 17, p. 15-20en_US
dc.subject251001 Oceanografía biológicaen_US
dc.subject.otherZooplanktonen_US
dc.subject.otherRespiratory Electron Transport System activity (ETS)en_US
dc.titleModification of the electron transport system (ETS) method for routine measurements of respiratory rates of zooplanktonen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.2989/025776196784158446
dc.identifier.scopus0030420569-
dc.identifier.isi000168876300002-
dcterms.isPartOfSouth African Journal Of Marine Science-Suid-Afrikaanse Tydskrif Vir Seewetenskap-
dcterms.sourceSouth African Journal Of Marine Science-Suid-Afrikaanse Tydskrif Vir Seewetenskap[ISSN 0257-7615],v. 17, p. 15-20-
dc.contributor.authorscopusid7401734371-
dc.contributor.authorscopusid57192268038-
dc.contributor.authorscopusid6701465678-
dc.description.lastpage20-
dc.description.firstpage15-
dc.relation.volume17-
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid1273639-
dc.contributor.daisngid1665494-
dc.contributor.daisngid489706-
dc.identifier.investigatorRIDL-9561-2014-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Gomez, M
dc.contributor.wosstandardWOS:Torres, S
dc.contributor.wosstandardWOS:Hernandez-Leon, S
dc.date.coverdateEnero 1996
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptEcofisiología de Organismos Marinos-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Biología-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptOceanografía Biológica y Cambio Global-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptDepartamento de Biología-
crisitem.author.orcid0000-0002-7396-6493-
crisitem.author.orcid0000-0002-3085-4969-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.fullNameGómez Cabrera, María Milagrosa-
crisitem.author.fullNameTorres Curbelo, Santiago-
crisitem.author.fullNameHernández León, Santiago Manuel-
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