|Title:||Interleukin-1β stimulates sphingomyelin hydrolysis in cultured granulosa cells: Evidence for a regulatory role of ceramide on progesterone and prostaglandin biosynthesis||Other Titles:||Interleukin-1 beta stimulates sphingomyelin hydrolysis in cultured granulosa cells: Evidence for a regulatory role of ceramide on progesterone and prostaglandin biosynthesis||Authors:||Santana, Pino
Ruiz De Galarreta, Carlos M.
Fanjul, Luisa F.
|UNESCO Clasification:||320502 Endocrinología||Keywords:||Hormone-Induced Differentiation
Rat Preovulatory Follicles
Receptor Antagonist, et al
|Issue Date:||1996||Publisher:||0013-7227||Journal:||Endocrinology (Philadelphia)||Abstract:||In granulosa cells labeled to isotopic steady-state with [H-3]serine, addition of interleukin-1 beta (IL1 beta) or bacterial sphingomyelinase (SMase) induced a rapid decrease (similar to 60% by 10 min) in cellular [H-3]Sphingomyelin content and a prolonged generation (up to 60 min) of [H-3]ceramide, the immediate lipid-moiety generated in response to sphingomyelin hydrolysis.In FSH-treated cells, IL1 beta (0.3-30 ng-/ml) inhibited progesterone biosynthesis in a dose-dependent manner, an effect that was also observed in cells expose to increasing concentrations of bacterial SMase (0.003-0.3 U/ml) or the membrane-permeable ceramide an analogue N-hexanoylsphingosine (C6-cer:0.1-10 mu M). Abrogation of progesterone biosynthesis was not a sole consequence of inadequate cAMP biosynthesis because cyclic nucleotide levels remained elevated (3- to 4-fold. over untreated cultures) after addition of IL1 beta, SMase, or two different cell permeable ceramide analogues (C2-cer and C6-cer) to gonadotropin-stimulated granulosa cells. Moreover, taken into account that exogenous SMase or C6-cer partially abolished progesterone biosynthesis induced by But(2)cAMP (0.5 mM) or cholera toxin (CTX: 1 mu g/ml), the above mentioned results support the notion that activation of the sphingomyelin pathway exerts its inhibitory effects on granulosa cell steroidogenic activity at site(s) of action both proximal and distal to cAMP generation.As determined by RT-PCR analysis, the inhibitory effect of IL1 beta, SMase or C6-cer on gonadotropin-stimulated steroidogenesis was accompanied by arrested transcription of the mitochondrial cholesterol side chain cleavage enzyme (P450scc) and 3 beta-hydroxysteroid dehydrogenase/Delta 5-4 isomerase, the two FSH-inducible steps involved in progesterone biosynthesis.Although bacterial SMase or the ceramide analogue C6-cer alone did not exactly reproduce the effect of IL1 beta on granulosa cell prostaglandin E(2) (PGE(2)) biosynthesis, both agents augmented net PGE(2) production and messenger RNA levels of the inducible prostaglandin endoperoxide synthase/cyclooxygenase (PGHS-2) in cytokine-trated cells.Although the effect on PGHS-2 messenger RNA may account for the facilitatory role of ceramide on IL1 beta-induced PGE(2) biosynthesis, neither SMase nor the membrane-permeant ceramide analogue were able to augment prostaglandin accumulation in the presence of exogenously added arachidonate precursor.Collectively, whereas these results show that ceramide triggers a negative-effector pathway that is both necessary and sufficient to reproduce the inhibitory effect of IL1 beta on FSH-stimulated granulosa cell steroidogenesis, they also support the notion that sphingomyelin hydrolysis may be important for cytokine-induced PGHS-2 expression but not sufficient to reproduce IL1 beta-stimulated PGE(2) biosynthesis.||URI:||http://hdl.handle.net/10553/48296||ISSN:||0013-7227||DOI:||10.1210/endo.137.6.8641202||Source:||Endocrinology[ISSN 0013-7227],v. 137 (6), p. 2480-2489|
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