Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/48296
DC FieldValueLanguage
dc.contributor.authorSantana, Pinoen_US
dc.contributor.authorLlanes, Luzen_US
dc.contributor.authorHernández, Inmaculadaen_US
dc.contributor.authorGonzalez-Robayna, Ignacioen_US
dc.contributor.authorTabraue, Carlosen_US
dc.contributor.authorGonzalez-Reyes, Juanen_US
dc.contributor.authorQuintana, Joséen_US
dc.contributor.authorEstevez, Franciscoen_US
dc.contributor.authorRuiz De Galarreta, Carlos M.en_US
dc.contributor.authorFanjul, Luisa F.en_US
dc.contributor.otherTabraue, Carlos-
dc.contributor.otherEstevez, Francisco-
dc.contributor.otherQuintana, Jose-
dc.contributor.otherGonzalez Robayna, Ignacio-
dc.contributor.otherHernandez Gonzalez, Inmaculada-
dc.date.accessioned2018-11-23T20:31:15Z-
dc.date.available2018-11-23T20:31:15Z-
dc.date.issued1996en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10553/48296-
dc.description.abstractIn granulosa cells labeled to isotopic steady-state with [H-3]serine, addition of interleukin-1 beta (IL1 beta) or bacterial sphingomyelinase (SMase) induced a rapid decrease (similar to 60% by 10 min) in cellular [H-3]Sphingomyelin content and a prolonged generation (up to 60 min) of [H-3]ceramide, the immediate lipid-moiety generated in response to sphingomyelin hydrolysis.In FSH-treated cells, IL1 beta (0.3-30 ng-/ml) inhibited progesterone biosynthesis in a dose-dependent manner, an effect that was also observed in cells expose to increasing concentrations of bacterial SMase (0.003-0.3 U/ml) or the membrane-permeable ceramide an analogue N-hexanoylsphingosine (C6-cer:0.1-10 mu M). Abrogation of progesterone biosynthesis was not a sole consequence of inadequate cAMP biosynthesis because cyclic nucleotide levels remained elevated (3- to 4-fold. over untreated cultures) after addition of IL1 beta, SMase, or two different cell permeable ceramide analogues (C2-cer and C6-cer) to gonadotropin-stimulated granulosa cells. Moreover, taken into account that exogenous SMase or C6-cer partially abolished progesterone biosynthesis induced by But(2)cAMP (0.5 mM) or cholera toxin (CTX: 1 mu g/ml), the above mentioned results support the notion that activation of the sphingomyelin pathway exerts its inhibitory effects on granulosa cell steroidogenic activity at site(s) of action both proximal and distal to cAMP generation.As determined by RT-PCR analysis, the inhibitory effect of IL1 beta, SMase or C6-cer on gonadotropin-stimulated steroidogenesis was accompanied by arrested transcription of the mitochondrial cholesterol side chain cleavage enzyme (P450scc) and 3 beta-hydroxysteroid dehydrogenase/Delta 5-4 isomerase, the two FSH-inducible steps involved in progesterone biosynthesis.Although bacterial SMase or the ceramide analogue C6-cer alone did not exactly reproduce the effect of IL1 beta on granulosa cell prostaglandin E(2) (PGE(2)) biosynthesis, both agents augmented net PGE(2) production and messenger RNA levels of the inducible prostaglandin endoperoxide synthase/cyclooxygenase (PGHS-2) in cytokine-trated cells.Although the effect on PGHS-2 messenger RNA may account for the facilitatory role of ceramide on IL1 beta-induced PGE(2) biosynthesis, neither SMase nor the membrane-permeant ceramide analogue were able to augment prostaglandin accumulation in the presence of exogenously added arachidonate precursor.Collectively, whereas these results show that ceramide triggers a negative-effector pathway that is both necessary and sufficient to reproduce the inhibitory effect of IL1 beta on FSH-stimulated granulosa cell steroidogenesis, they also support the notion that sphingomyelin hydrolysis may be important for cytokine-induced PGHS-2 expression but not sufficient to reproduce IL1 beta-stimulated PGE(2) biosynthesis.en_US
dc.languageengen_US
dc.publisher0013-7227-
dc.relation.ispartofEndocrinology (Philadelphia)en_US
dc.sourceEndocrinology[ISSN 0013-7227],v. 137 (6), p. 2480-2489en_US
dc.subject320502 Endocrinologíaen_US
dc.subject.otherHormone-Induced Differentiationen_US
dc.subject.otherRat Preovulatory Folliclesen_US
dc.subject.otherTumor-Necrosis-Factoren_US
dc.subject.otherEndoperoxide Synthaseen_US
dc.subject.otherReceptor Antagonisten_US
dc.subject.otherSignal Transductionen_US
dc.subject.otherMessenger-Rnaen_US
dc.subject.otherH Synthaseen_US
dc.subject.otherExpressionen_US
dc.subject.otherCytokinesen_US
dc.titleInterleukin-1β stimulates sphingomyelin hydrolysis in cultured granulosa cells: Evidence for a regulatory role of ceramide on progesterone and prostaglandin biosynthesisen_US
dc.title.alternativeInterleukin-1 beta stimulates sphingomyelin hydrolysis in cultured granulosa cells: Evidence for a regulatory role of ceramide on progesterone and prostaglandin biosynthesisen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1210/endo.137.6.8641202en_US
dc.identifier.scopus9344265205-
dc.identifier.isiA1996UM80200038-
dcterms.isPartOfEndocrinology-
dcterms.sourceEndocrinology[ISSN 0013-7227],v. 137 (6), p. 2480-2489-
dc.contributor.authorscopusid7003526778-
dc.contributor.authorscopusid7007111159-
dc.contributor.authorscopusid7102922509-
dc.contributor.authorscopusid6507425244-
dc.contributor.authorscopusid6506833060-
dc.contributor.authorscopusid16749890800-
dc.contributor.authorscopusid8681043500-
dc.contributor.authorscopusid7003810011-
dc.contributor.authorscopusid7003806034-
dc.contributor.authorscopusid7004158812-
dc.description.lastpage2489en_US
dc.identifier.issue6-
dc.description.firstpage2480en_US
dc.relation.volume137en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:A1996UM80200038-
dc.contributor.daisngid31818872-
dc.contributor.daisngid329218-
dc.contributor.daisngid161507-
dc.contributor.daisngid7087446-
dc.contributor.daisngid2446020-
dc.contributor.daisngid2922182-
dc.contributor.daisngid4816905-
dc.contributor.daisngid27693644-
dc.contributor.daisngid521026-
dc.contributor.daisngid128315-
dc.contributor.daisngid384944-
dc.contributor.daisngid1664323-
dc.contributor.daisngid1127140-
dc.identifier.investigatorRIDD-7126-2015-
dc.identifier.investigatorRIDK-5125-2014-
dc.identifier.investigatorRIDK-5709-2014-
dc.identifier.investigatorRIDK-9671-2014-
dc.identifier.investigatorRIDK-7776-2014-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Santana, P-
dc.contributor.wosstandardWOS:Llanes, L-
dc.contributor.wosstandardWOS:Hernandez, I-
dc.contributor.wosstandardWOS:GonzalezRobayna, I-
dc.contributor.wosstandardWOS:Tabraue, C-
dc.contributor.wosstandardWOS:GonzalezReyes, J-
dc.contributor.wosstandardWOS:Quintana, J-
dc.contributor.wosstandardWOS:Estevez, F-
dc.contributor.wosstandardWOS:DeGalarreta, CMR-
dc.contributor.wosstandardWOS:Fanjul, LF-
dc.date.coverdateJunio 1996en_US
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptMedio Ambiente y Salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0001-8937-9034-
crisitem.author.orcid0000-0002-7650-4454-
crisitem.author.orcid0000-0001-9920-8116-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameHernández González, Inmaculada Servanda-
crisitem.author.fullNameGonzález Robayna, Ignacio Javier-
crisitem.author.fullNameTabraue Tarbay, Carlos-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
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