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http://hdl.handle.net/10553/48293
Título: | Regulation by ceramide of epidermal growth factor signal transduction and mitogenesis in cell lines overexpressing the growth factor receptor | Autores/as: | Gallardo Campos, Germán Tabraue, C. Quintana, J López Blanco, Félix Cabrera, J. Díaz, R. Estévez, F. Ruiz de Galarreta, C. M. Fanjul Rodríguez, Luisa Fernanda Santana, P. |
Clasificación UNESCO: | 32 Ciencias médicas 2407 Biología celular |
Palabras clave: | Activated Protein-Kinase Tumor-Necrosis-Factor Egf-Receptor Induced Apoptosis Tyrosine Kinase, et al. |
Fecha de publicación: | 2000 | Publicación seriada: | Cellular and Molecular Biology | Resumen: | Ceramide has emerged as a pleiotropic signal mediator of cellular responses including differentiation, proliferation, cell cycle arrest and apoptosis. In the present study we evaluated the effect of cell permeant ceramide analogues on ligand-induced tyrosine phosphorylation of the EGF receptor (EGFR), phospholipase C gamma (PLC gamma) activity and cell proliferation. Treatment with N-acetylsphingosine (C2-cer) and N-hexanoylceramide (C6-cer) prevented EGF-induced tyrosine trans-phosphorylation of the receptor in two different cell lines overexpressing the human EGFR (A431 and EGF-T17 cells). In contrast, treatment of A431 and EGFR-T17 cells with C2-cer or C6-cer did not affect the ligand binding capacity of the receptor, an effect that was however: observed after TPA-induced activation of PKC. In addition EGF-stimulated PLC gamma activity was transiently decreased in A431 cells treated with C6-cer and only a modest, albeit significant reduction on ligand-induced H-3-InsP(3) generation was observed in EGFR-T17 cells pretreated with ceramide. We also examined the effect of C2-cer on serum (A431)- or EGF (EGFR-T17)-induced cell proliferation Treatment of EGFR-T17 cells with C2-cer (0.1-10 muM) did not affect cell viability, but prevented EGF-induced H-3-thymidine incorporation in a dose-dependent manner. In contrast, H-3-thymidine incorporation in serum-stimulated A431 cells decreased only at the higher doses of C2-cer used (1-10 muM), being this effect accompanied by a slight, albeit significant (20-25%), reduction in cell viability. | URI: | http://hdl.handle.net/10553/48293 | ISSN: | 0145-5680 | Fuente: | Cellular and molecular biology (Noisy-le-Grand, France), [ISSN 0145-5680], v. 46, p. 1305-1312, (Enero 2020). |
Colección: | Artículos |
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